Identification and characterization of an oocyte factor required for sperm decondensation in pig
文献类型： 外文期刊
作者： Li, Jingyu 1 ; Huan, Yanjun 2 ; Xie, Bingteng 1 ; Wang, Jiaqiang 1 ; Zhao, Yanhua 1 ; Jiao, Mingxia 1 ; Huang, Tianqing 1 ; Kong, Qingran 1 ; Liu, Zhonghua 1 ;
作者机构： 1.Northeast Agr Univ, Coll Life Sci, Lab Embryo Biotechnol, Harbin 150030, Heilongjiang Pr, Peoples R China
2.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan 250100, Shandong, Peoples R China
期刊名称：REPRODUCTION （ 影响因子：3.906； 五年影响因子：4.51 ）
ISSN： 1470-1626
年卷期： 2014 年 148 卷 4 期
页码：
收录情况： SCI
摘要： Mammalian oocytes possess factors to support fertilization and embryonic development, but knowledge on these oocyte-specific factors is limited. In the current study, we demonstrated that porcine oocytes with the first polar body collected at 33 h of in vitro maturation sustain IVF with higher sperm decondensation and pronuclear formation rates and support in vitro development with higher cleavage and blastocyst rates, compared with those collected at 42 h (P<0.05). Proteomic analysis performed to clarify the mechanisms underlying the differences in developmental competence between oocytes collected at 33 and 42 h led to the identification of 18 differentially expressed proteins, among which protein disulfide isomerase associated 3 (PDIA3) was selected for further study. Inhibition of maternal PDIA3 via antibody injection disrupted sperm decondensation; conversely, overexpression of PDIA3 in oocytes improved sperm decondensation. In addition, sperm decondensation failure in PDIA3 antibody-injected oocytes was rescued by dithiothreitol, a commonly used disulfide bond reducer. Our results collectively report that maternal PDIA3 plays a crucial role in sperm decondensation by reducing protamine disulfide bonds in porcine oocytes, supporting its utility as a potential tool for oocyte selection in assisted reproduction techniques.
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