Attenuation of a recombinant Marek's disease virus lacking the meq oncogene and evaluation on its immune efficacy against Marek's disease virus
文献类型： 外文期刊
作者： Sun, Peng 1 ; Cui, Ning 3 ; Liu, Linqing 5 ; Su, Shuai 1 ; Cheng, Ziqiang 1 ; Chen, Ruiai 6 ; Li, Yanpeng 7 ; Cui, Zhizho 1 ;
作者机构： 1.Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China
2.Shandong Agr Univ, Coll Vet Med, Tai An 271018, Shandong, Peoples R China
3.Shandong Acad Agr Sci, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Shandong, Peoples R China
4.Shandong Acad Agr Sci, Inst Anim Husb & Vet, Jinan 250100, Shandong, Peoples R China
5.China Anim Dis Control Ctr, China 100125, Peoples R China
6.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China
7.Zhaoqing Inst Biotechnol Co Ltd, Zhaoqing 526238, Guangdong, Peoples R China
关键词： Marek's disease virus; meq gene; cell culture passage; attenuation; immune efficacy
期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）
ISSN：
年卷期： 2020 年 99 卷 4 期
页码：
收录情况： SCI
摘要： SC9-2 is a recombinant Marek's disease virus (MDV) strain lacking the meq oncogene. Previous study demonstrated that SC9-2 virus provides good protection against challenge with a very virulent MDV rMd5, but it induces immunosuppressive effects in specific pathogen-free (SPF) chickens. In the present study, SC9-2 was serially passaged on chicken embryo fibroblast (CEF) cell cultures. The pathogenicity and immune efficacy of SC9-2/10th and SC9-2/40th against rMd5 were evaluated. Animal experimental results showed that SC9-2/10th and SC9-2/40th showed no lethality or tumorigenicity in SPF chickens. Body weight of chickens inoculated with SC9-2/40th were significantly higher than that of the chickens inoculated with SC9-2/10th but lower than that of the uninoculated controls. The severity of bursa and thymus atrophy (BTA) and spleen enlargement in SC9-2/40th-inoculated chickens were also weaker than the SC9-2/10th-inoculated ones but stronger than the uninoculated controls. Chickens inoculated with SC9-2/40th and SC9-2/10th showed similar antibody levels induced by H9N2 subtype avian influenza virus/Newcastle disease virus inactivated vaccines, both of which were lower than the uninoculated controls. Replication of SC9-2/40th was significantly lower than SC9-2/10th in feather follicle epithelium (FFE) of infected chickens. The immune protection index of SC9-2/40th was also lower than that of SC9-2/10th, but the difference was not significantly, and both of which were significant higher than that of the commercial MDV vaccine CVI988/Rispens. The results of our studies demonstrated that SC9-2/40th showed weaker severity of BTA, spleen enlargement, and body weight loss and lower replication level in FFE than SC9-2/10th in SPF chickens. However, SC9-2/40th was able to confer better immune protection as compared with CVI988/Rispens vaccination in SPF chickens. In conclusion, serially attenuation of SC9-2 in CEFs reduced the lymphoid organ atrophy and replication in SPF chickens, and the immune protective efficacy of attenuated viruses was still superior than CVI988/Rispens.
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