Increased Horizontal Transmission of Recombinant Marek's Disease Virus Due to Reticuloendotheliosis Virus Long Terminal Repeat Is the Major Competitive Advantage of the Virus Being a Prevalent Strain
文献类型： 外文期刊
作者： Su, Shuai 1 ; Cui, Ning 3 ; Li, Yanpeng 4 ; Yu, Meng Xin 1 ; Zhang, Ting 1 ; Cheng, Ziqiang 1 ; Chai, Jiaqian 1 ; Cui, Zh 1 ;
作者机构： 1.Shandong Agr Univ, Coll Vet Med, Tai An, Shandong, Peoples R China
2.Shandong Agr Univ, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An, Shandong, Peoples R China
3.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan, Shandong, Peoples R China
4.Zhaoqing Inst Biotechnol Co Ltd, Zhaoqing, Peoples R China
5.South China Agr Univ, Coll Vet Med, Guangzhou, Guangdong, Peoples R China
关键词： Marek's disease virus; REV LTR; horizontal transmission; competitive advantage; virus gene transcription
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2019 年 10 卷
页码：
收录情况： SCI
摘要： GX0101 is the first field Marek's disease virus (MDV) recombinant with an REV LTR insert isolated in China. We speculated that there was a selective advantage of GX0101 becoming the more prevalent field strain from a very low percentage of recombinant virus. In the study, dual fluorescence quantitative real-time PCR (DF-qPCR) that detects GX0101 and GX0101 Delta LTR simultaneously was established based on the genomic difference of GX0101 and its LTR deletion strain GX0101 Delta LTR. MDV natural transmission was simulated in specific-pathogen-free (SPF) chicks, and continuous tracking of GX0101 and GX0101 Delta LTR in chicks was carried out. The results showed that GX0101 possessed high horizontal transmission capacity, which could infect SPF chicks by contact in a short time and became the predominant strain following contact infections in chicken flocks. GX0101 still had a more significant advantage of horizontal transmission than GX0101 Delta LTR after continuous passage even if the initially infectious dose was significantly lower. There were 72 differentially expressed MDV genes between GX0101 and GX0101 Delta LTR, with the genes and gene products mainly involved in virus replication, tegument protein, glycoprotein, nucleocapsid protein, immune evasion, tumor development and/or pathogenesis, and hypothetical protein. Sixteen genes related to virus replication and transmission were significantly up-regulated. This is the first study to illuminate that increased horizontal transmission of recombinant MDV due to REV LTR was the competitive advantage of the virus being a prevalent strain and define the differential transcription profile of viral genes between GX0101 and GX0101 Delta LTR. This will be helpful for in-depth study on the molecular mechanism of increased horizontal transmission of MDV by REV LTR.
- 相关文献
作者其他论文 更多>>
-
Avian leukosis virus usurps the cellular SERBP1 protein to enhance its transcription and promote productive infections in avian cells
作者：Cui, Ning;Han, Xiaoxia;Yang, Xiao;Zhao, Xiaoran;Huang, Qinghua;Xu, Chuantian;Cui, Ning;Han, Xiaoxia;Yang, Xiao;Zhao, Xiaoran;Huang, Qinghua;Xu, Chuantian;Cui, Ning;Han, Xiaoxia;Yang, Xiao;Zhao, Xiaoran;Huang, Qinghua;Xu, Chuantian;Yang, Xiao;Zhao, Xiaoran;Su, Shuai;Yang, Xiao;Zhao, Xiaoran;Su, Shuai;Han, Xiaoxia
关键词：avian leukosis virus; U3; mutation; SERBP1; interaction
-
Co-infection of H9N2 subtype avian influenza virus and QX genotype live attenuated infectious bronchitis virus increase the pathogenicity in SPF chickens
作者：Huang, Qinghua;Yang, Xiao;Zhao, Xiaoran;Han, Xiaoxia;Sun, Shouli;Xu, Chuantian;Cui, Ning;Huang, Qinghua;Yang, Xiao;Zhao, Xiaoran;Han, Xiaoxia;Sun, Shouli;Xu, Chuantian;Cui, Ning;Huang, Qinghua;Yang, Xiao;Zhao, Xiaoran;Han, Xiaoxia;Sun, Shouli;Xu, Chuantian;Cui, Ning;Zhao, Xiaoran;Han, Xiaoxia;Yang, Xiao;Zhao, Xiaoran;Han, Xiaoxia;Lu, Mei
关键词：Avian influenza virus; Live attenuated infectious bronchitis virus; Co -infection; Pathogenicity; Proteomic
-
Wheat Pm55 alleles exhibit distinct interactions with an inhibitor to cause different powdery mildew resistance
作者：Lu, Chuntian;Du, Jie;Chen, Heyu;Jin, Yinyu;Meng, Xiangru;Zhang, Ting;Xing, Liping;Kong, Lingna;Chen, Peidu;Cao, Aizhong;Zhang, Ruiqi;Lu, Chuntian;Du, Jie;Chen, Heyu;Jin, Yinyu;Meng, Xiangru;Zhang, Ting;Fu, Bisheng;Xing, Liping;Wu, Jizhong;Cao, Aizhong;Zhang, Ruiqi;Gong, Shuangjun;Fu, Bisheng;Wu, Jizhong;Molnar, Istvan;Molnar, Istvan;Holusova, Katerina;Said, Mahmoud;Dolezel, Jaroslav;Said, Mahmoud;Li, Genying
关键词：
-
Integrated metabolome and transcriptome analyses of anthocyanin biosynthesis reveal key candidate genes involved in colour variation of Scutellaria baicalensis flowers
作者：Guo, Fengdan;Guan, Renwei;Sun, Xinru;Zhang, Cuicui;Cui, Ning;Wang, Ping;Lin, Huibin;Shan, Chenggang;Liu, Mengyu
关键词：Scutellaria baicalensis; Flower colour; Anthocyanin; Structural genes; Transcription factors
-
Molecular characterization and pathogenicity study of a highly pathogenic strain of chicken anemia virus that emerged in China
作者：Fang, Lichun;Qi, Lihong;Jia, Huiyue;Wang, Yixin;Cui, Zhizhong;Zhao, Peng;Hu, Yuhang
关键词：chicken anemia virus; immunosuppressive virus; severe anemia; molecular characteristics; pathogenicity
-
Divergent effects of moderate grazing duration on carbon sequestration between temperate and alpine grasslands in China
作者：Zhang, Zhenchao;Zhao, Yanhua;Sun, Juan;Hua, Ting;Li, Yanpeng;Wang, Yi;Wang, Fei;Sun, Jian
关键词：Moderate grazing; Grazing duration; Carbon sequestration; Grassland type; Meta-analysis
-
Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously
作者：Cui, Ning;Sun, Shiping;Sun, Shouli;Xu, Chuantian;Hrabchenko, Nataliia;Huang, Qinghua;Cui, Ning;Sun, Shiping;Sun, Shouli;Xu, Chuantian;Hrabchenko, Nataliia;Huang, Qinghua;Sun, Shiping;Su, Shuai;Lu, Mei
关键词：fowl adenovirus; mix infection; high-throughput; genome; evolution
