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Determination of Lactoferrin in Camel Milk by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry Using an Isotope-Labeled Winged Peptide as Internal Standard

文献类型： 外文期刊

作者： Li, Xia 1 ; Li, Zengmei 1 ; Xu, Enmin 2 ; Chen, Ling 2 ; Feng, Hua 1 ; Chen, Lu 1 ; Deng, Ligang 1 ; Guo, Dongliang 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, 202 Gongyebeilu Rd, Jinan 250100, Shandong, Peoples R China

2.Shandong Vet Drug Qual Inspect Inst, 68 Huaicun St, Jinan, Shandong, Peoples R China

关键词： lactoferrin; camel milk; ultrahigh-performance liquid chromatography-tandem mass spectrometry; heparin affinity chromatography

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期： 2019 年 24 卷 22 期

页码：

收录情况： SCI

摘要： An ultrahigh-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of lactoferrin in camel milk based on the signature peptide. The camel lactoferrin was purified by heparin affinity chromatography and then used to screen tryptic signature peptides. The signature peptide was selected on the basis of sequence database search and identified from the tryptic hydrolysates of purified camel lactoferrin by ultrahigh-performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry. The pretreatment procedures included the addition of isotope-labeled winged peptide and the disposal of lipids and caseins followed by an enzymatic digestion with trypsin. Analytes were separated on an Acquity UPLC BEH 300 C18 column and then detected on a triple-quadrupole mass spectrometer in 7 min. The limits of detection and quantification were 3.8 mg kg(-1) and 11 mg kg(-1), respectively. The recoveries ranged from 74.5% to 103.6%, with relative standard deviations below 7.7%. The validated method was applied to determine the lactoferrin in ten samples collected from Xinjiang Province.

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