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Development and validation of an immunogold chromatographic test for on-farm detection of PRRSV

文献类型： 外文期刊

作者： Zhou, Sheng-hua 1 ; Cui, Shang-jin 1 ; Chen, Chang-mu 1 ; Zhang, Fan-chao 1 ; Li, Jun 2 ; Zhou, Shun 3 ; Oh, Jin-Sik 1 ;

作者机构： 1.Chinese Acad Agr Sci, Div Swine Infect Dis, State Key Lab Vet Biotechnol, Harbin Vet Res Inst, Harbin 15001, Peoples R China

2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China

3.Qingdao Agr Univ, Qingdao 262509, Peoples R China

关键词： Immunochromatographic test strip;Porcine reproductive and respiratory syndrome virus

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

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收录情况： SCI

摘要： An immunochromatographic test strip was developed to detect porcine reproductive and respiratory syndrome virus (PRRSV). The test uses two gold-labeled monoclonal antibodies: D5 against recombinant nucleocapsid protein (rN) and E9 against recombinant M protein (rM). In the test, PRRSV binds to a mixture of D5 and E9 labeled with colloidal gold; the complexes move through a membrane and are captured by rabbit anti-rM and anti-rN antibodies at a test line, producing a reddish-purple band because of the increased concentration of gold. Unbound monoclonal antibodies move past the test line to be captured by goat anti-mouse antibodies, producing a band at a control line. In samples without PRRSV or with low virus concentration, a band appears only at the control line. A crossover-trial demonstrated that the test strip was highly specific for PRRSV. The test strip detection limit was between 7.8 x 10(3) and 1.6 x 10(4) TCID50/ml. Analysis of 100 clinical samples indicated that the sensitivity, specificity. and accuracy of the immunochromatographic test strip relative to reverse transcription polymerase chain reaction (RT-PCR) were 97.0, 93.9, and 96.0%. respectively. Because the test is simple and rapid, it can be used by an unskilled person to detect PRRSV in the field.

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[1]Development and validation of reverse transcription loop-mediated isothermal amplification for detection of PRRSV. Chen, Changmu,Cui, Shangjin,Zhang, Chaofan,Li, Jun,Wang, Jinbao. 2010

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