Extraction, purification and anticancer activity studies on triterpenes from pomegranate peel
文献类型： 外文期刊
作者： Zhang, Mengqi 1 ; Zhang, Yutao 1 ; Guo, Xu 1 ; Chen, Yingying 1 ; Li, Huimin 4 ; Zhou, Guihua 4 ; Sun, Shutao 1 ; Ren, Qidong 1 ; Simal-Gandara, Jesus 5 ; Sun, Jinyue 1 ; Li, Ningyang 3 ; Liu, Chao 1 ;
作者机构： 1.Shandong Acad Agr Sci, Key Lab Novel Food Resources Proc, Key Lab Agroprod Proc Technol Shandong Prov, Minist Agr & Rural Affairs,Inst Agrofood Sci & Tec, 23788 Gongye North Rd, Jinan 250100, Peoples R China
2.Shandong Huatai Ind Technol Inst Nutr & Hlth Co Lt, 9 Hongji Rd, Jinan 251400, Peoples R China
3.Shandong Agr Univ, Coll Food Sci & Engn, Key Lab Food Proc Technol & Qual Control Shandong, Tai An 271017, Peoples R China
4.Dev Ctr Melon Fruit & Vegetable Ind Kashi, Kashi 844000, Peoples R China
5.Univ Vigo, Fac Sci, Dept Analyt Chem & Food Sci, Nutr & Bromatol Grp, E-32004 Orense, Spain
6.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
期刊名称：FOOD & FUNCTION （ 影响因子：6.1； 五年影响因子：6.5 ）
ISSN： 2042-6496
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Pomegranate peel is the by-product of pomegranate processing, which contains a lot of triterpene compounds. In this study, the total triterpenes of pomegranate peel (TPP) were extracted using an ultrasonic-assisted ethanol extraction method under optimal conditions, purified using D-101 macroporous resin to obtain a purity of 75.28%. The triterpenes in TPP were mainly pentacyclic triterpenes determined by LC-MS/MS. Network pharmacological analysis predicted that the anticancer targets were closely related to the MAPK pathway. The in vitro results showed that TPP could inhibit cell proliferation, promote apoptosis, reduce mitochondrial membrane potential and increase ROS levels. The western blot results indicated that the expression levels of the apoptotic proteins Bax, Bcl-2, cytochrome C, cleaved caspase-3 and cleaved caspase-9 were increased. In addition, the protein expression of the MAPK pathway predicted by network pharmacology also changed significantly. These results provided that TPP has potential for adjuvant therapy of tumors. This study is the first to enrich and prepare triterpenes from pomegranate peel, as well as study the anti-tumor mechanism by network pharmacology, which has great significance for the treatment of cancer and high-value use of pomegranate peel.
- 相关文献
作者其他论文 更多>>
-
Anti-Tumor Activity and Mechanism of Silibinin Based on Network Pharmacology and Experimental Verification
作者：Li, Peihai;Wang, Dexu;Yang, Xueliang;Liu, Changyu;Li, Xiaobin;Zhang, Xuanming;Liu, Kechun;Zhang, Yun;Wang, Rongchun;Zhang, Mengqi;Wang, Changyun
关键词：silibinin; anti-tumor; network pharmacology; adenoid cystic carcinoma; Western blot analysis
-
Marine-Fungus-Derived Natural Compound 4-Hydroxyphenylacetic Acid Induces Autophagy to Exert Antithrombotic Effects in Zebrafish
作者：Xin, Shaoshuai;Li, Peihai;Wang, Lizhen;Zhang, Xuanming;Zhang, Shanshan;Li, Xiaobin;Liu, Kechun;Zhang, Mengqi;Mu, Zhenqiang;Mu, Zhenqiang;Lin, Houwen
关键词：marine-derived fungus; 4-hydroxyphenylacetic acid; antithrombotic; autophagy
-
Effect of Lactobacillus salivarius SNK-6 on egg quality, intestinal morphology, and cecal microbial community of laying hens
作者：Liu, Wei;Liu, Jie;Li, Dapeng;Han, Haixia;Sun, Yan;Lei, Qiuxia;Wang, Jie;Zhou, Yan;Cao, Dingguo;Li, Huimin;Li, Fuwei;Liu, Wei;Liu, Jie;Li, Dapeng;Han, Haixia;Sun, Yan;Lei, Qiuxia;Wang, Jie;Zhou, Yan;Cao, Dingguo;Li, Huimin;Li, Fuwei;Liu, Wei;Liu, Jie;Li, Dapeng;Han, Haixia;Sun, Yan;Lei, Qiuxia;Wang, Jie;Zhou, Yan;Cao, Dingguo;Li, Huimin;Li, Fuwei;Yan, Huaxiang
关键词：Lactobacillus salivarius; egg quality; intestinal morphology; cecal microbial community; laying hen
-
MUP1 mediates urolithin A alleviation of chronic alcohol-related liver disease via gut-microbiota-liver axis
作者：Zhang, Hongbo;Li, Chaoyue;Han, Lin;Xiao, Yao;Liu, Zhigang;Wang, Min;Bian, Ji;Liu, Chao;Gong, Lan
关键词：Alcohol-related liver disease; urolithin A; endoplasmic reticulum stress; MUP1 protein; gut-microbiota-liver axis
-
The molecular mechanism of action for the potent antitumor component extracted using supercritical fluid extraction from Croton crassifolius root
作者：Guo, Xu;Zhang, Rui-Rui;Sun, Jin-Yue;Chen, Ying-Ying;Sun, Hui;Liu, Chao;Liu, Yan;Yuan, Xian-Shun
关键词：Anti-proliferative; Apoptosis induction; Molecular mechanism; Croton crassifolius
-
Nutrition and flavor analysis of Spirulina through co-fermentation with Lactobacillus acidophilus and Kluyveromyces marxianus and its effect on attenuating metabolic associated fatty liver disease
作者：Yu, Jinhui;Liu, Chao;Liu, Yuanyuan;Ma, Deyuan;Chen, Gao;Han, Fuwen;You, Shengbo;Bi, Yuping;Wu, Yingjie;Yu, Jinhui;Ma, Deyuan;Chen, Gao;You, Shengbo;Bi, Yuping;Liu, Chao;Wang, Mingjie;Wang, Mingjie;Liu, Yuanyuan;Ran, Liyuan;Yu, Zichao;Wu, Yingjie
关键词：Spirulina; Lactobacillus acidophilus; Kluyveromyces marxianus; Co -fermentation; MAFLD
-
Preparation of emulsions-filled sodium alginate hydrogel beads to enhance the anti-cancer activity of fangchinoline in vivo and in vitro
作者：Liu, Chao;Wang, Mu-Xuan;Lyu, Xu-Tao;Guo, Xu;Sun, Jin-Yue;Lu, Chao-Xia;Li, Ning-Yang;Zhang, Kai;Prieto, M. A.
关键词：Fangchinoline; Emulsions; Hydrogel beads; Anti-cancer; Molecular mechanism
