A visual bio-barcode immunoassay for sensitive detection of triazophos based on biochip silver staining signal amplification
文献类型： 外文期刊
作者： Wang, Yuanshang 1 ; Du, Pengfei 2 ; Abd El-Aty, Am 3 ; Chen, Ge 1 ; Jia, Huiyan 5 ; Cui, Xueyan 1 ; Oz, Emel 6 ; Zhang, Y 1 ;
作者机构： 1.Chinese Acad Agr Sci, Minist Agr, Key Lab Agroprod Qual & Safety, Inst Qual Stand & Testing Technol Agroprod,Key La, Beijing 100081, Peoples R China
2.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Key Lab Agroprod Proc Technol Shandong Prov, Key Lab Novel Food Resources Proc,Minist Agr, Jinan 250100, Peoples R China
3.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
4.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkey
5.Ningbo Acad Agr Sci, Ningbo 315040, Peoples R China
6.Ataturk Univ, Fac Agr, Dept Food Engn, TR-25240 Erzurum, Turkey
7.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res Inst, Nanning 530007, Peoples R China
8.Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA
9.Univ Calif Davis, UC Davis Comprehens Canc Ctr, Davis, CA 95616 USA
关键词： Bio-barcode; Biochip silver staining; Immunoassay; Triazophos; Gold nanoparticles
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2021 年 347 卷
页码：
收录情况： SCI
摘要： Herein, a novel visual method for detecting triazophos based on competitive bio-barcode immunoassay was described. The competitive immunoassay was established by gold nanoparticles (AuNPs), magnetic microparticle (MMPs) and triazophos, combined with biochip hybridization system to detect the residual of triazophos in water and apple. Because AuNPs carried many bio-barcodes, which hybridized with labeled DNA on the biochip, catalyzed signal amplification using silver staining was detected by grayscale values as well as the naked eye. Notably, the grayscale values decreases with increasing the concentrations of triazophos, and the color change weakened gradually. The detection range was in between 0.05 and 10 ng/mL and the minimum detection limit was set at 0.04 ng/mL. Percent recovery calculated from spiked water and apple samples ranged between 55.4 and 107.8% with relative standard deviations (RSDs) of 12.4-24.9%. It has therefore been shown that this protocol provides a new insight for rapid detection of small molecule pesticides in various matrices.
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