Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method
文献类型： 外文期刊
作者： Zhang, Hao 1 ; Yao, Yuan 1 ; Chen, Zhi 2 ; Sun, Wenbo 2 ; Liu, Xiang 3 ; Chen, Lei 4 ; Sun, Jianhai 3 ; Qiu, Xianbo 1 ; Yu, Duli 1 ; Zhang, Lulu 1 ;
作者机构： 1.Beijing Univ Chem Technol, Coll Informat Sci & Technol, Beijing 100029, Peoples R China
2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China
3.Chinese Acad Sci, Aerosp Informat Res Inst, Beijing 100094, Peoples R China
4.Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Anim Resistance Biol, Jinan 250014, Peoples R China
关键词： African swine fever virus (ASFV); loop-mediated isothermal amplification (LAMP); surface plasmon resonance (SPR); fluorescence detection
期刊名称：BIOSENSORS-BASEL （ 影响因子：5.519； 五年影响因子：5.313 ）
ISSN：
年卷期： 2022 年 12 卷 4 期
页码：
收录情况： SCI
摘要： African swine fever (ASF) is a swine disease with a very high fatality rate caused by a complex double-stranded DNA virus. The fluorescence PCR detection method is widely used for virus nucleic acid detection. Surface plasmon resonance (SPR) is a label-free and real-time detection method, unlike the fluorescence PCR detection method. In this research, we detected the loop-mediated isothermal amplification (LAMP) products of the African swine fever virus by using the SPR and fluorescence methods separately and simultaneously. By comparing the positive and negative control results, we found that the SPR response unit is completely different before and after the LAMP process. In addition, the fluorescence results on a chip showed that with an increase in the concentration of the sample, the cycle threshold (CT) value decreased, which is consistent with commercial instruments. Both the decline rate of the SPR response unit and the CT value of the fluorescence realized were used to distinguish the positive control from the negative control and water, which indicates that the SPR method can be combined with fluorescence to detect LAMP products. This research provides a label-free and simple method for detecting LAMP products.
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