Detection and identification of SARS-CoV-2 and influenza a based on microfluidic technology
文献类型： 外文期刊
作者： Liu, Yujie 1 ; Yu, Guanliu 1 ; Liang, Hongkun 1 ; Sun, Wenbo 2 ; Zhang, Lulu 3 ; Mauk, Michael G. 4 ; Li, Hua 1 ; Chen, Lei 1 ;
作者机构： 1.Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Anim Resistance Biol, Jinan, Shandong, Peoples R China
2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan, Shandong, Peoples R China
3.Beijing Univ Chem Technol, Coll Informat Sci & Technol, Beijing, Peoples R China
4.Univ Penn, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USA
期刊名称：ANALYTICAL METHODS （ 影响因子：2.7； 五年影响因子：2.7 ）
ISSN： 1759-9660
年卷期： 2024 年 16 卷 27 期
页码：
收录情况： SCI
摘要： As of now, the global COVID-19 pandemic caused by SARS-CoV-2, which began in 2019, has been effectively controlled. However, the symptoms of influenza A virus infection were similar to those of SARS-CoV-2 infection, but they required different treatment approaches. To make the detection more accurate and the treatment more targeted. We developed a system that integrates RPA and CRISPR assays, allowing for the rapid, highly specific, and sensitive detection and differentiation of SARS-CoV-2, H1N1, and H3N2. Under isothermal amplification conditions, the RPA-CRISPR Cas12a detection system achieved a detection limit as low as 5 copies per mu L, demonstrating excellent specificity. The measurement time was approximately 30 minutes. The RPA-CRISPR Cas12a detection system combined with the microfluidic chip we designed to simultaneously detect three viruses, providing a potential solution for efficient and reliable diagnosis. We developed a system that integrates RPA-CRISPR Cas12a with microfluidic chip, allowing for the rapid, highly specific, and sensitive detection and differentiation of SARS-CoV-2, H1N1, and H3N2.
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