文献类型： 外文期刊

作者： Zhang, Meng-Qi ¹ ; Sun, Ke-Xin ² ; Guo, Xu ¹ ; Chen, Ying-Ying ¹ ; Feng, Cai-Yun ¹ ; Chen, Jia-Shu ¹ ; Barreira, Joao C. M. ³ ; Prieto, Miguel A. ⁴ ; Sun, Jin-Yue ¹ ; Zhang, Jian-Dong ⁵ ; Li, Ning-Yang ⁷ ; Liu, Chao ¹ ;

作者机构： 1.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Key Lab Novel Food Resources Proc, Key Lab Agroprod Proc Technol Shandong Prov,Minist, 23788 Gongye North Rd, Jinan 250100, Peoples R China

2.Shandong Agr Univ, Coll Food Sci & Engn, Key Lab Food Proc Technol & Qual Control Shandong, Tai An 271018, Peoples R China

3.Inst Politecn Braganca, Ctr Invest Montanha CIMO, Campus Santa Apolonia, P-5300253 Braganca, Portugal

4.Univ Vigo, Fac Sci, Dept Analyt Chem & Food Sci, Nutr & Bromatol Grp, E32004, Orense, Spain

5.Shandong First Med Univ, Dept Oncol, Shandong Key Lab Rheumat Dis & Translat Med, Affiliated Hosp 1, Jinan 250014, Peoples R China

6.Shandong Prov Qianfoshan Hosp, Shandong Key Lab Rheumat Dis & Translat Med, Jinan 250014, Peoples R China

7.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

关键词： Astragali Radix; Hyperuricemia; UA Transporter; Mechanism

期刊名称：JOURNAL OF ETHNOPHARMACOLOGY （ 影响因子：5.4； 五年影响因子：5.3 ）

ISSN： 0378-8741

年卷期： 2023 年 317 卷

页码：

收录情况： SCI

摘要： Ethnopharmacological relevance: Astragali Radix (AR) is the dry root of the leguminous plants Astragalus membranaceus (Fisch) Beg. var. mongholicus (Beg) Hsiao, and Astragalus membranaceus (Fisch) Bge., being used as a medicinal and edible resource. AR is used in traditional Chinese medicine prescriptions to treat hyperuricemia, but this particular effect is rarely reported, and the associated mechanism of action is still need to be elucidated.Aim of the study: To research the uric acid (UA)-lowering activity and mechanism of AR and the representative compounds through the constructed hyperuricemia mouse and cellular models.Materials and methods: In our study, the chemical profile of AR was analysed by UHPLC-QE-MS, as well as the mechanism of action of AR and the representative compounds on hyperuricemia was studied through the constructed hyperuricemia mouse and cellular models.Results: The main compounds in AR were terpenoids, flavonoids and alkaloids. Mice group treated with the highest AR dosage showed significantly lower (p < 0.0001) serum uric acid (208 & PLUSMN; 9 & mu;mol/L) than the control group (317 & PLUSMN; 11 & mu;mol/L). Furthermore, UA increased in a dose-dependence manner in urine and faeces. Serum creatinine and blood urea nitrogen standards, as well as xanthine oxidase in mice liver, decreased (p < 0.05) in all cases, indicating that AR could relieve acute hyperuricemia. UA reabsorption protein (URAT1 and GLUT9) was down-regulated in AR administration groups, while the secretory protein (ABCG2) was up-regulated, indicating that AR could promote the excretion of UA by regulating UA transporters via PI3K/Akt signalling pathway.Conclusion: This study validated the activity, and revealed the mechanism of AR in reducing UA, which provided experimental and clinical basis for the treatment of hyperuricemia with it.