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Cloning and Characterization of 5 ' Flanking Regulatory Sequences of AhLEC1B Gene from Arachis Hypogaea L.

文献类型： 外文期刊

作者： Tang, Guiying 1 ; Xu, Pingli 1 ; Liu, Wei 2 ; Liu, Zhanji 1 ; Shan, Lei 1 ;

作者机构： 1.Shandong Acad Agr Sci, Biotech Res Ctr, Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan 250100, Peoples R China

2.Shandong Univ, Coll Agr, Jinan 250100, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 10 期

页码：

收录情况： SCI

摘要： LEAFY COTYLEDON1 (LEC1) is a B subunit of Nuclear Factor Y (NF-YB) transcription factor that mainly accumulates during embryo development. We cloned the 50 flanking regulatory sequence of AhLEC1B gene, a homolog of Arabidopsis LEC1, and analyzed its regulatory elements using online software. To identify the crucial regulatory region, we generated a series of GUS expression frameworks driven by different length promoters with 5' terminal and/or 3' terminal deletion. We further characterized the GUS expression patterns in the transgenic Arabidopsis lines. Our results show that both the 65bp proximal promoter region and the 52bp 5' UTR of AhLEC1B contain the key motifs required for the essential promoting activity. Moreover, AhLEC1B is preferentially expressed in the embryo and is co-regulated by binding of its upstream genes with both positive and negative corresponding cis-regulatory elements.

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