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Analysis of the Amino Acids of Soy Globulins by AOT Reverse Micelles and Aqueous Buffer

文献类型： 外文期刊

作者： Zhao, Xiaoyan 1 ; Chen, Jun 1 ; Lu, Zhifang 2 ; Ling, Xiangqing 1 ; Deng, Peng 1 ; Zhu, Qingjun 1 ; Du, Fangling 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Jinan 250100, Peoples R China

2.Southeast Univ, Affiliated Hosp 2, Dept Orthoped, Nanjing 210003, Peoples R China

关键词： Reverse micelles; Soybean; 7S and 11S globulins; Amino acids

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

ISSN： 0273-2289

年卷期： 2011 年 165 卷 3-4 期

页码：

收录情况： SCI

摘要： The 7S and 11S globulins from soybean proteins using reverse micelle and aqueous buffer extraction methods were characterized by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and scanning electron microscope (SEM), and their amino acid compositions were also evaluated. SDS-PAGE did not show electrophoretic differences between 7S and 11S globulin subunits with two extraction methods. SEM analysis showed that the AOT reverse micelle processing of 7S and 11S globulins induced a reduction of droplet size. Some individual amino acid contents of 7S and 11S globulins using two extraction methods were different, some were similar. In all the samples, the glutamic acid, aspartic acid, and leucine were the dominant amino acids while the cystine and methionine were the first-limiting amino acids. The proportion of essential amino acids to the total amino acids (E/T) of the 7S globulin from aqueous buffer and reverse micelles was similar. While significant differences were obtained in the proportion of E/T of the 11S globulin.

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