Drought tolerance through over-expression of the expansin gene TaEXPB23 in transgenic tobacco
文献类型: 外文期刊
作者: Li, Feng 1 ; Xing, Shichao 1 ; Guo, Qifang 1 ; Zhao, Meirong 1 ; Zhang, Jin 1 ; Gao, Qiang 1 ; Wang, Guiping 2 ; Wang, We 1 ;
作者机构: 1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Shandong Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
2.Shandong Inst Pomol, Tai An 271000, Shandong, Peoples R China
关键词: malondialdehyde: MDA;542-78-9;mRNA: expression;photosynthetic rate;water stress;drought tolerance;electrolyte leakage;biochemical parameter;physiological parameter;plant coleoptile growth
期刊名称:JOURNAL OF PLANT PHYSIOLOGY ( 影响因子:3.549; 五年影响因子:4.164 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Expansins are proteins that are the key regulators of wall extension during plant growth. To investigate the role of TaEXPB23, a wheat expansin gene, we analyzed TaEXPB23 mRNA expression levels in response to water stress in wheat and examined the drought resistance of transgenic tobaccos over-expressing TaEXPB23. We found that the expression of TaEXPB23 corresponded to wheat coleoptile growth and the response to water stress. The results also indicated that the transgenic tobacco lines lost water more slowly than the wild-type (WT) plants under drought stress; their cells could sustain a more integrated structure under water stress than that of WT. Other physiological and biochemical parameters under water stress, such as electrolyte leakage, malondialdehyde (MDA) level, photosynthetic rate, F-v/F-m and Phi PSII, also suggested that the transgenic tobaccos were more drought resistant than WT plants
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