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4-Coumarate-CoA ligase (4-CL) enhances flavonoid accumulation, lignin synthesis, and fruiting body formation in Ganoderma lucidum

文献类型： 外文期刊

作者： Meng, Li 1 ; Zhou, Ruyue 1 ; Liang, Lidan 1 ; Zang, Xizhe 1 ; Lin, Jialong 1 ; Wang, Qingji 1 ; Wang, Li 1 ; Wang, Wei 1 ; Li, Zhuang 1 ; Ren, Pengfei 2 ;

作者机构： 1.Shandong Agr Univ, Coll Plant Protect, Shandong Prov Key Lab Agr Microbiol, Tai An 271018, Peoples R China

2.Shandong Acad Agr Sci, State Key Lab Nutrient Use & Management, Jinan 250100, Peoples R China

3.Shandong Acad Agr Sci, Key Lab Wastes Matrix Utilizat, Minist Agr & Rural Affairs, Jinan 250100, Peoples R China

关键词： Medicinal fungi; 4-coumarate-CoA ligase; Secondary metabolites; Primordia formation

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2024 年 899 卷

页码：

收录情况： SCI

摘要： It is now understood that 4-Coumarate-CoA ligases (4-CL) are pivotal in bridging the phenylpropanoid metabolic pathway and the lignin biosynthesis pathway in plants. However, limited information on 4-CL genes and their functions in fungi is available. In this study, we cloned the 4-CL gene (Gl21040) from Ganoderma lucidum, which spans 2178 bp and consists of 10 exons and 9 introns. We also developed RNA interference and overexpression vectors for Gl21040 to investigate its roles in G. lucidum. Our findings indicated that in the Gl21040 interference transformants, 4-CL enzyme activities decreased by 31 %-57 %, flavonoids contents decreased by 10 %-22 %, lignin contents decreased by 20 %-36 % compared to the wild-type (WT) strain. Conversely, in the Gl21040 overexpression transformants, 4-CL enzyme activity increased by 108 %-143 %, flavonoids contents increased by 8 %-37 %, lignin contents improved by 15 %-17 % compared to the WT strain. Furthermore, primordia formation was delayed by approximately 10 days in the Gl21040-interferenced transformants but occurred 3 days earlier in the Gl21040-overexpressed transformants compared to the WT strain. These results underscored the involvement of the Gl21040 gene in flavonoid synthesis, lignin synthesis, and fruiting body formation in G. lucidum.

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