Multicolor aptasensors for pesticide multiresidues detection in agricultural products using bioorthogonal surface-enhanced Raman scattering tags
文献类型： 外文期刊
作者： Yan, Mengmeng 1 ; Wang, Hao 1 ; Li, Min 1 ; Zhang, Wenjun 1 ; Du, Hongxia 1 ; Chen, Zilei 1 ; Zhu, Chao 1 ; She, Yongxin 3 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Peoples R China
2.Shandong Prov Key Lab Test Technol Food Qual & Saf, Jinan 250100, Peoples R China
3.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
关键词： Multiplex analysis; Matrix effect; Biologically Raman-silent region; 4-Ethenylbenzenamine; 2-Amino-4-cyanopyridine; Prussian blue
期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.4 ）
ISSN： 0039-9140
年卷期： 2023 年 265 卷
页码：
收录情况： SCI
摘要： Realizing accurate pesticide multiresidue detection in a complex matrix is still a challenge for point-of-care sensing methods. Herein, we introduced background-free and multicolor aptasensors based on bioorthogonal surface-enhanced Raman scattering (SERS) tags and successfully applied them to analyze multiple pesticide residues. The excellent anti-interference and multiplex capability are due to the application of three bioorthogonal Raman reporters involving 4-ethenylbenzenamine (4-EBZM), Prussian blue (PB) and 2-amino-4-cyanopyridine (AMCP) with alkynyl and cyano groups, which demonstrated apparent Raman shift peaks at 1993 cm-1, 2160 cm-1, and 2264 cm-1 in the biologically Raman-silent region, respectively. Ultimately, a detection range of 1-50 nM for acetamiprid, atrazine and malathion was achieved with detection limits of 0.39, 0.57 and 0.16 nM, respectively. The developed aptasensors were successfully used to determine pesticide residues in real samples. These proposed multicolor aptasensors offer an effective strategy for pesticide multiresidue detection with advantages of anti-interference, high specificity and high sensitivity.
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