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Self-Responsive Fluorescence Aptasensor for Lactoferrin Determination in Dairy Products

文献类型： 外文期刊

作者： Liu, Hao 1 ; Gao, Xibao 1 ; Qin, Hongwei 2 ; Yan, Mengmeng 2 ; Zhu, Chao 2 ; Li, Linsen 4 ; Qu, Feng 4 ;

作者机构： 1.Shandong Univ, Cheeloo Coll Med, Sch Publ Hlth, Dept Phys & Chem Inspect, Jinan 250000, Peoples R China

2.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Peoples R China

3.Shandong Prov Key Lab Test Technol Food Qual & Saf, Jinan 250100, Peoples R China

4.Beijing Inst Technol, Sch Life Sci, Key Lab Mol Med & Biotherapy, 5 South Zhongguancun St, Beijing 100081, Peoples R China

关键词： aptamer; self-responsive; fluorescence aptasensor; lactoferrin detection

期刊名称：MOLECULES （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN：

年卷期： 2024 年 29 卷 13 期

页码：

收录情况： SCI

摘要： In this study, a self-responsive fluorescence aptasensor was established for the determination of lactoferrin (Lf) in dairy products. Herein, the aptamer itself functions as both a recognition element that specifically binds to Lf and a fluorescent signal reporter in conjunction with fluorescent moiety. In the presence of Lf, the aptamer preferentially binds to Lf due to its specific and high-affinity recognition by folding into a self-assembled and three-dimensional spatial structure. Meanwhile, its reduced spatial distance in the aptamer-Lf complex induces a FRET phenomenon based on the quenching of 6-FAM by amino acids in the Lf protein, resulting in a turn-off of the fluorescence of the system. As a result, the Lf concentration can be determined straightforwardly corresponding to the change in the self-responsive fluorescence signal. Under the optimized conditions, good linearities (R-2 > 0.99) were achieved in an Lf concentration range of 2 similar to 10 mu g/mL for both standard solutions and the spiked matrix, as well as with the desirable detection limits of 0.68 mu g/mL and 0.46 mu g/mL, respectively. Moreover, the fluorescence aptasensor exhibited reliable recoveries (89.5-104.3%) in terms of detecting Lf in three commercial samples, which is comparable to the accuracy of the HPCE method. The fluorescence aptasensor offers a user-friendly, cost-efficient, and promising sensor platform for point-of-need detection.

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