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Drought and Heat Stress-Mediated Modulation of Alternative Splicing in the Genes Involved in Biosynthesis of Metabolites Related to Tea Quality

文献类型： 外文期刊

作者： Ding, Y. Q. 1 ; Fan, K. 1 ; Wang, Y. 1 ; Fang, W. P. 2 ; Zhu, X. J. 2 ; Chen, L. 3 ; Sun, L. T. 1 ; Qiu, C. 1 ; Ding, Z. T. 1 ;

作者机构： 1.Shandong Acad Agr Sci, Tea Res Inst, Rizhao 276800, Peoples R China

2.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China

3.Chinese Acad Agr Sci, Tea Res Inst, Haikou 310008, Hainan, Peoples R China

关键词： tea; Camellia sinensis (L.); O. Kuntze; alternative splicing; amino acids; flavonoids; volatile compounds

期刊名称：MOLECULAR BIOLOGY （ 影响因子：1.374； 五年影响因子：1.266 ）

ISSN： 0026-8933

年卷期： 2022 年 56 卷 2 期

页码：

收录情况： SCI

摘要： Alternative splicing (AS) regulates mRNAs at the post-transcriptional level to affect both their amounts and the protein function. However, little is known about the roles of AS in regulation of biosynthesis of amino acids, flavonoids, and volatile compounds in tea plants. In this study, we used Iso-seq and transcriptome deep sequencing (RNA-seq) to identify AS events, and analyzed the expression of respective mRNAs in tea plants under drought (DS), heat stress (HS), and their combination (HD). By RT-PCR, we validated the AS events in nine genes involved in the biosynthesis of amino acids and flavonoids. The genes accumulating AS transcripts under DS, HS, and HD conditions included those encoding for anthocyanidin reductase (ANR), dihydrofavonol-4-reductase-like (DFRA), and chalcone isomerase (CHI). Similarly, genes directly or indirectly involved in the biosynthesis of volatile compounds such as lipoxygenase (LOX), terpenoid/terpene synthase (TPS), and 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) also had AS events. Our study revealed that AS might specifically regulate the biosynthesis of amino acids in tea plants under stressful conditions. Moreover, we suggest that the AS events within the ANR and DFRA transcripts might play an important role in the regulation of flavonoid biosynthesis under DS, HS, and HD conditions. This study improved our understanding of the genetic drivers of the changes in the content of bioactive ingredients of tea plants subjected to abiotic stresses.

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