Peanut (Arachis hypogaea L.) S-adenosylmethionine decarboxylase confers transgenic tobacco with elevated tolerance to salt stress
文献类型： 外文期刊
作者： Meng, D. -Y. 1 ; Yang, S. 1 ; Xing, J. -Y. 2 ; Ma, N. -N. 3 ; Wang, B. -Z. 2 ; Qiu, F. -T. 2 ; Guo, F. 1 ; Meng, J. 1 ; Zhang, J. -l. 1 ; Wan, S. -B. 4 ; Li, X. -G. 1 ;
作者机构： 1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan, Peoples R China
2.Linyi Univ, Coll Life Sci, Linyi, Shandong, Peoples R China
3.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An, Shandong, Peoples R China
4.Shandong Prov Key Lab Crop Genet Improvement Ecol, Jinan, Peoples R China
关键词： Peanut (Arachis hypogaea L; ); polyamines; reactive oxygen species; salt stress; SAMDC; transgenic tobacco
期刊名称：PLANT BIOLOGY （ 影响因子：3.081； 五年影响因子：2.972 ）
ISSN： 1435-8603
年卷期： 2021 年 23 卷 2 期
页码：
收录情况： SCI
摘要： Polyamines play an important role in stress response. In the pathway of polyamines synthesis, S-adenosylmethionine decarboxylase (SAMDC) is one of the key enzymes. In this study, a full length cDNA of SAMDC (AhSAMDC) was isolated from peanut (Arachis hypogaea L.). Phylogenetic analysis revealed high sequence similarity between AhSAMDC and SAMDC from other plants. In peanut seedlings exposed to sodium chloride (NaCl), the transcript level of AhSAMDC in roots was the highest at 24 h that decreased sharply at 72 and 96 h after 150 mM NaCl treatment. However, the expression of AhSAMDC in peanut leaves was significantly inhibited, and the transcript levels in leaves were not different compared with control These results implied the tissue-specific and time-specific expression of AhSAMDC. The physiological effects and functional mechanism of AhSAMDC were further evaluated by overexpressing AhSAMDC in tobaccos. The transgenic tobacco lines exhibited higher germination rate and longer root length under salt stress. Reduced membrane damage, higher antioxidant enzyme activity, and higher proline content were also observed in the transgenic tobacco seedlings. What's more, AhSAMDC also led to higher contents of spermidine and spermine, which can help to scavenge reactive oxygen species. Together, this study suggests that AhSAMDC enhances plant resistance to salt stress by improving polyamine content and alleviating membrane damage
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