Effects of ectopically expressed hyperthermophilic archaeon (Pyrococcus furiosus) ribulose-1,5-bisphosphate carboxylase/oxygenase on tobacco photosynthesis
文献类型： 外文期刊
作者： Li, X. -G. 1 ; Yang, J. 3 ; Wang, R. 3 ; Tang, X. -F. 4 ; Meng, J. -J. 1 ; Qin, H. -J. 5 ; Liu, X. 6 ; Guo, F. 1 ; Wan, S. -B. 1 ;
作者机构： 1.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan 250100, Peoples R China
2.Shandong Prov Key Lab Genet Improvement Ecol & Ph, Jinan 250100, Peoples R China
3.Natl Tobacco Corp, Zhengzhou Tobacco Res Inst China, Zhengzhou 450001, Peoples R China
4.Wuhan Univ, Coll Life Sci, Wuhan 430212, Peoples R China
5.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
6.Chinese Acad Sci, Inst Genet & Dev Biol, State Key L
关键词： high-temperature stress; Nicotiana benthamiana; photosynthesis; Rubisco; virus-mediated ectopic expression
期刊名称：PHOTOSYNTHETICA （ 影响因子：3.189； 五年影响因子：3.38 ）
ISSN： 0300-3604
年卷期： 2013 年 51 卷 3 期
页码：
收录情况： SCI
摘要： Pyrococcus furiosus is a hyperthermophilic archaeon. Its ribulose-1,5-bisphosphate carboxylase/oxygenase (PfRubisco) has only large subunit (L). PfRubisco has a novel (L-2)(5), decameric structure and it possesses higher carboxylase activity and thermotolerance. To assess the potential functionality of PfRubisco in higher plants under high-temperature stress, PfRubisco coding sequence was transiently expressed in Nicotiana benthamiana by Pea early browning virus mediated ectopic expression. The transgenic PfRubisco plants produced chlorotic yellow stripes in their leaves. Relative to the control leaves, those with yellow stripes exhibited decreased net photosynthetic rate and chlorophyll content, altered chloroplast ultrastructure, and more severe photoinhibition of both photosystem I and II. We concluded that the ectopic expression of PfRubisco might disrupt the chloroplast development and function in N. benthamiana. The potential cause of the disruption was discussed.
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