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Comparative proteomic investigation of drought responses in foxtail millet

文献类型： 外文期刊

作者： Pan, Jiaowen 1 ; Li, Zhen 1 ; Wang, Qingguo 1 ; Garrell, Anna K. 6 ; Liu, Min 5 ; Guan, Yanan 3 ; Zhou, Wenqing; Liu, Wei 1 ;

作者机构： 1.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China

2.Key Lab Genet Improvement Ecol & Physiol Crops, Jinan 250100, Shandong, Peoples R China

3.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Shandong, Peoples R China

4.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Shandong, Peoples R China

5.Shandong Agr & Engn Univ, Jinan 250100, Shandong, Peoples R China

6.Boragen Inc, Durham, NC 27709

关键词： Foxtail millet (Setariaitalica L; ); Drought stress; Comparative proteomics; Expression pattern; Western blot; qRT-PCR

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2018 年 18 卷

页码：

收录情况： SCI

摘要： BackgroundFoxtail millet (Setaria italica L. P. Beauv) has been considered as a tractable model crop in recent years due to its short growing cycle, lower amount of repetitive DNA, inbreeding nature, small diploid genome, and outstanding abiotic stress-tolerance characteristics. With modern agriculture facing various adversities, it's urgent to dissect the mechanisms of how foxtail millet responds and adapts to drought and stress on the proteomic-level.ResultsIn this research, a total of 2474 differentially expressed proteins were identified by quantitative proteomic analysis after subjecting foxtail millet seedlings to drought conditions. 321 of these 2474 proteins exhibited significant expression changes, including 252 up-regulated proteins and 69 down-regulated proteins. The resulting proteins could then be divided into different categories, such as stress and defense responses, photosynthesis, carbon metabolism, ROS scavenging, protein synthesis, etc., according to Gene Ontology annotation. Proteins implicated in fatty acid and amino acid metabolism, polyamine biosynthesis, hormone metabolism, and cell wall modifications were also identified. These obtained differential proteins and their possible biological functions under drought stress all suggested that various physiological and metabolic processes might function cooperatively to configure a new dynamic homeostasis in organisms. The expression patterns of five drought-responsive proteins were further validated using western blot analysis. The qRT-PCR was also carried out to analyze the transcription levels of 21 differentially expressed proteins. The results showed large inconsistency in the variation between proteins and the corresponding mRNAs, which showed once again that post-transcriptional modification performs crucial roles in regulating gene expression.ConclusionThe results offered a valuable inventory of proteins that may be involved in drought response and adaption, and provided a regulatory network of different metabolic pathways under stress stimulation. This study will illuminate the stress tolerance mechanisms of foxtail millet, and shed some light on crop germplasm breeding and innovation.

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[1]Comparative proteomic investigation of drought responses in foxtail millet. Pan, Jiaowen,Li, Zhen,Wang, Qingguo,Liu, Wei,Pan, Jiaowen,Li, Zhen,Wang, Qingguo,Liu, Wei,Guan, Yanan,Guan, Yanan,Liu, Wei,Liu, Min,Garrell, Anna K.,Zhou, Wenqing. 2018

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