A chimeric PCV rescued virus with the immunogenic cap gene of PCV3 cloned into the genomic backbone of the nonpathogenic PCV1 induces specific antibodies but with no pathogenic in pigs
文献类型： 外文期刊
作者： Shi, Jianli 1 ; Wu, Xiaoyan 1 ; Wang, Shuo 3 ; Li, Jiaxin 4 ; Wang, Zhao 6 ; Yang, Ying 2 ; Peng, Zhe 1 ; Liu, Chang 1 ; Xu, Shaojian 1 ; Li, Chen 1 ; Tian, Yao 4 ; Han, Hong 1 ; Nataliia 1 ; Li, Jun 1 ; Wang, Yongming 5 ; Han, Xianjie 4 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan, Peoples R China
2.Shandong Normal Univ, Coll Life Sci, Jinan, Peoples R China
3.Shandong Univ, Coll Life Sci, Qingdao, Peoples R China
4.Qingdao Agr Univ, Qingdao, Peoples R China
5.Shandong Huahong Biol Engn CO LTD, Binzhou, Peoples R China
6.Shandong First Med Univ & Shandong Acad Med Sci, Sch Lab Anim, Jinan, Peoples R China
7.Shandong First Med Univ & Shandong Acad Med Sci, Shandong Lab Anim Ctr, Jinan, Peoples R China
8.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, 202, Ind North Rd, Jinan 250100, Peoples R China
关键词： Porcine circovirus virus type 3; Chimeric virus; Rescue; Viral neutralization; Vaccine candidate
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.8； 五年影响因子：4.0 ）
ISSN： 0882-4010
年卷期： 2022 年 173 卷
页码：
收录情况： SCI
摘要： A chimeric PCV called PCV1-3 with the immunogenic Cap gene of pathogenic PCV type 3(PCV3) cloned into the genomic skeleton of the nonpathogenic PCV1 was rescued and inoculated into PCV3 negative piglets. The results of fluorescence quantitative PCR showed that the PCV1-3 DNA detected in serum and tissues was negative. The pathogenicity of piglets showed that PCV 1-3 did not cause the clinical characteristics and pathological changes. The viral neutralization assay revealed that infected pigs could produce antibodies and neutralize the viral ac-tivity. All results showed that chimeric virus induced specific antibodies but with no pathogenic in pigs, which provided new candidate strains for the development of PCV3 vaccine.
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