文献类型： 外文期刊
作者： Xu, Xiao 1 ; You, Yinwei 1 ; Zhang, Long 1 ;
作者机构： 1.China Agr Univ, Dept Entomol, Beijing, Peoples R China
2.Shandong Acad Agr Sci, Biotech Res Ctr, Jinan, Shandong, Peoples R China
关键词： Neuroscience; Issue 125; odorant receptor; RNA in situ hybridization; neuron; sensillum; insect antennae
期刊名称：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS （ 影响因子：1.355； 五年影响因子：1.696 ）
ISSN： 1940-087X
年卷期： 2017 年 125 期
页码：
收录情况： SCI
摘要： Insects have evolved sophisticated olfactory reception systems to sense exogenous chemical signals. These chemical signals are transduced by Olfactory Receptor Neurons (ORNs) housed in hair-like structures, called chemosensilla, of the antennae. On the ORNs' membranes, Odorant Receptors (ORs) are believed to be involved in odor coding. Thus, being able to identify genes localized to the ORNs is necessary to recognize OR genes, and provides a fundamental basis for further functional in situ studies. The RNA expression levels of specific ORs in insect antennae are very low, and preserving insect tissue for histology is challenging. Thus, it is difficult to localize an OR to a specific type of sensilla using RNA in situ hybridization. In this paper, a detailed and highly effective RNA in situ hybridization protocol particularly for lowly expressed OR genes of insects, is introduced. In addition, a specific OR gene was identified by conducting double-color fluorescent in situ hybridization experiments using a co-expressing receptor gene, Orco, as a marker.
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