Genome organization and variation in the 3 '-partial sequence of garlic latent virus in China
文献类型： 外文期刊
作者： Chen, J 1 ; Zheng, HY 2 ; Chen, JP 3 ; Yang, CL 4 ;
作者机构： 1.Zhejiang Acad Agr Sci, Dept Virol, Hangzhou 310021, Peoples R China
2.Zhejiang Univ, Coll Life Sci, Hangzhou 310029, Peoples R China
3.Huazhong Agr Univ, Dept Plant Protect, Wuhan 430070, Peoples R China
4.Shandong Acad Agr Sci, Dept Plant Protect, Jinan 250100, Peoples R China
关键词： garlic latent virus;nucleotide sequence;genome organization;variation
期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）
ISSN： 1006-9305
年卷期： 2002 年 45 卷 4 期
页码：
收录情况： SCI
摘要： Ten different isolates of a carlavirus were detected by degenerate PCR from 12 garlic samples collected from 6 provinces in China, and the complete genome sequence of the Zhejiang isolate ZJ1 and 3'-terminal sequences of 9 other isolates were determined. The RNA genome of isolate ZJ1 consisted of 8363nts excluding the 3'-poly (A) tail, and the genome organization was similar to other carlaviruses with 6 open reading frames encoding a replicase, TGB1, TGB2, TGB3, CP and NABP respectively. Sequence comparisons showed that all 10 isolates were Garlic latent virus (GarLV). The variations in the TGB2, TGB3 and NABP were more significant than those in the CP. High homology was also detected between those isolates and Shallot latent virus (ShLV). Phylogenetic analysis suggested that GarLV isolates from garlic can be divided into 4 main groups and Chinese isolates belonged to each group. This is the first reported molecular analysis of members of the genus Carlavirus in China.
- 相关文献
作者其他论文 更多>>
-
Biotechnology applied to garlic and onion
作者：Xu, PW;Yang, CL;Yang, CY
关键词：
-
A preliminary study on genetic analysis and purity assessment of the garlic germplasm and seed bulbs by the "finger printing" technique
作者：Xu, PW;Yang, CL;Qu, SS;Yang, CY
关键词：garlic;germplasm;RAPD;genetic;purity
-
Biotechnology applied to garlic and onion
作者：Xu, PW;Yang, CL;Yang, CY
关键词：
-
A preliminary study on genetic analysis and purity assessment of the garlic germplasm and seed bulbs by the "finger printing" technique
作者：Xu, PW;Yang, CL;Qu, SS;Yang, CY
关键词：garlic; germplasm; RAPD; genetic; purity
-
Characterisation of the welsh onion isolate of Shallot yellow stripe virus from China
作者：Chen, J;Wei, CB;Zheng, HY;Shi, YH;Adams, MJ;Lin, L;Zhang, QY;Wang, SJ;Chen, JP
关键词：
-
A virus related to Soybean mosaic virus from Pinellia ternata in China and its comparison with local soybean SMV isolates
作者：Chen, J;Zheng, HY;Lin, L;Adams, MJ;Antoniw, JF;Zhao, MF;Shang, YF;Chen, JP
关键词：
-
Inflorescence meristem culture and economic analysis of virus-free garlic (Allium sativum L.) in commercial production
作者：Xu, PW;Yang, CL;Qu, SH;Yang, CY;Srinives, P
关键词：propagation;seed garlic;bolting
