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Heterologous expression of two Glycine max omega-3 fatty acid desaturases in Saccharomyces cerevisiae

文献类型： 外文期刊

作者： Zhang, H. T. 1 ; Bi, Y. P. 1 ; Liu, Z. J. 1 ; Shan, L. 1 ;

作者机构： 1.Shandong Acad Agr Sci, High Tech Res Ctr, Key Lab Genet Improvement Crop Anim & Poultry Sha, Jinan 250100, Peoples R China

关键词： Glycine max;Saccharomyces cerevisiae;omega-3 fatty acid desaturase;alpha-linolenic acid

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）

ISSN： 1021-4437

年卷期： 2009 年 56 卷 4 期

页码：

收录情况： SCI

摘要： alpha-Linolenic acid (ALA, C 18:3(Delta 9,12,15)) has many important biological functions. omega-3 Fatty acid desaturase (FAD3), existing in cytosolic and plastidic compartments of higher plants, catalyzes linoleic acid (LA) desaturation to produce ALA. GmFAD3A-2 and GmFAD3C genes encoding cytosolic FAD3 from Qihuang 29 soybean were cloned and inserted into p416 vector and expressed in K601 yeast strain. Gas chromatography showed that the transformed yeast strains could produce ALA. The ALA accumulation levels for the strains transformed with GmFAD3A-2 or GmFAD3C genes were 0.77 +/- 0.1 and 4.13 +/- 0.4% of total fatty acids, respectively, while, as compared with that of the control, the contents of LA decreased from 14.34 +/- 0.8 to 10.93 +/- 0.0 and 7.85 +/- 0.1%, respectively, implying that the GmFAD3C enzyme is more vigorous or stable, than GmFAD3A-2.

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