山东省农业科学院文献资源数据库平台

Cloning of Acyl-ACP thioesterase FatA from Arachis hypogaea L. and its expression in Escherichia coli

收藏
分享
全文链接

文献类型：外文期刊

作者： Chen, G. ¹ ; Peng, Z. Y. ¹ ; Xuan, N. ¹ ; Zhang, Y. ¹ ; Bi, Y. P. ¹ ;

作者机构： 1.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan, Peoples R China

2.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China

关键词： Acyl-acyl carrier protein thioesterases;rapid amplification of cDNA ends;fatty acid;peanut

期刊名称：RESEARCH JOURNAL OF BIOTECHNOLOGY （影响因子：0.233；五年影响因子：0.252 ）

ISSN： 0973-6263

年卷期： 2012 年 7 卷 4 期

页码：

收录情况： SCI

摘要： In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3'-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and showed 50-70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was expressed in all tissues of A. hypogaea L., but most strongly in the immature seeds harvested at 60 days after pegging. Heterologous expression of AhFatA in Escherichia coli affected bacterial growth and changed the fatty acid profiles of the membrane lipid, resulting in directed accumulation towards palmitoleic acid and oleic acid. These results indicate that AhFatA is at least partially responsible for determining the high palmitoleic acid and oleic acid composition of E. coli.

相关文献

[1]Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803. Chen, Gao,Zhang, Yan,Peng, Zhenying,Fan, Zhongxue,Bian, Fei,Yu, Jinhui,Chen, Gao,Zhang, Yan,Peng, Zhenying,Fan, Zhongxue,Bian, Fei,Yu, Jinhui,Chen, Jun,Qin, Song,Chen, Jun,He, Qingfang. 2017

[2]Functional expression analysis of an acyl-ACP thioesterase FatB1 from Arachis hypogaea L. seeds in Escherichia coli. Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,He, Qingfang,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping. 2012

[3]Transgenic Expression and Identification of Recombinant Human Proinsulin in Peanut. Zheng Ling,Wang Yu,Wan Shu-Bo,Peng Zhen-Ying,Bi Yu-Ping,Zheng Ling,Wang Yu,Bian Fei,Wan Shu-Bo,Peng Zhen-Ying,Zheng Ling,Wang Yu,Bian Fei,Wan Shu-Bo,Peng Zhen-Ying,Bi Yu-Ping,Jiao Qi-Qing,Qu Shu-Jie,Wan Shu-Bo,Bi Yu-Ping. 2016

[4]Transcriptome Analysis of Calcium and Hormone-Related Gene Expressions during Different Stages of Peanut Pod Development. Li, Yan,Meng, Jingjing,Yang, Sha,Guo, Feng,Zhang, Jialei,Geng, Yun,Cui, Li,Li, Xinguo,Wan, Shubo. 2017

[5]Calcium contributes to photoprotection and repair of photosystem II in peanut leaves during heat and high irradiance. Yang, Sha,Wang, Fang,Guo, Feng,Meng, Jing-Jing,Li, Xin-Guo,Yang, Sha,Guo, Feng,Meng, Jing-Jing,Li, Xin-Guo,Wan, Shu-Bo,Wang, Fang. 2015

[6]Genome-Wide Identification and Comparative Analysis of Cytosine-5 DNA Methyltransferase and Demethylase Families in Wild and Cultivated Peanut. Wang, Pengfei,Gao, Chao,Bian, Xiaotong,Zhao, Shuzhen,Zhao, Chuanzhi,Xia, Han,Song, Hui,Hou, Lei,Wan, Shubo,Wang, Xingjun. 2016

[7]Peanut (Arachis hypogaea L.) Omics and Biotechnology in China. Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Liu, Shuan-Tao. 2011

[8]Identification of Metabolites and Transcripts Involved in Salt Stress and Recovery in Peanut. Cui, Feng,Liu, Yiyang,Han, Yan,Wan, Shubo,Li, Guowei,Cui, Feng,Liu, Yiyang,Han, Yan,Wan, Shubo,Li, Guowei,Sui, Na,Liu, Shanshan,Duan, Guangyou. 2018

[9]Small RNA profiling and degradome analysis reveal regulation of microRNA in peanut embryogenesis and early pod development. Gao, Chao,Wang, Pengfei,Zhao, Shuzhen,Zhao, Chuanzhi,Xia, Han,Hou, Lei,Zhang, Ye,Li, Changsheng,Wang, Xingjun,Wang, Xingjun,Ju, Zheng. 2017

[10]Genome-Wide Dissection of the Heat Shock Transcription Factor Family Genes in Arachis. Wang, Pengfei,Song, Hui,Li, Changsheng,Li, Pengcheng,Li, Aiqin,Guan, Hongshan,Hou, Lei,Wang, Xingjun,Wang, Xingjun. 2017

[11]Identification and expression dynamics of three WUSCHEL related homeobox 13 (WOX13) genes in peanut. Wang, Pengfei,Li, Changsheng,Li, Cui,Zhao, Chuanzhi,Xia, Han,Zhao, Shuzhen,Hou, Lei,Gao, Chao,Wan, Shubo,Wang, Xingjun,Wang, Pengfei,Li, Changsheng,Li, Cui,Zhao, Chuanzhi,Xia, Han,Zhao, Shuzhen,Hou, Lei,Gao, Chao,Wan, Shubo,Wang, Xingjun.

[12]Comparative proteomics of peanut gynophore development under dark and mechanical stimulation. Sun, Yong,Wang, Qingguo,Li, Zhen,Hou, Lei,Liu, Wei,Dai, Shaojun,Sun, Yong.

[13]Transcriptome and Differential Expression Profiling Analysis of the Mechanism of Ca2+ Regulation in Peanut (Arachis hypogaea) Pod Development. Yang, Sha,Zhang, Jialei,Geng, Yun,Guo, Feng,Meng, Jingjing,Li, Xinguo,Li, Lin,Wang, Jianguo,Sui, Na,Wan, Shubo. 2017

[14]Physiological fundamentals of the AnM cultivation technique in peanut production: Leaf photosynthetic hysteresis is reduced by exposing hypocotyls. Qin, Feifei,Takano, Tetsuo,Qin, Feifei,Xu, Hui-lian,Qin, Feifei. 2012

[15]Identification of QTLs associated with oil content and mapping FAD2 genes and their relative contribution to oil quality in peanut (Arachis hypogaea L.). Pandey, Manish K.,Qiao, Lixian,Feng, Suping,Khera, Pawan,Wang, Hui,Guo, Baozhu,Pandey, Manish K.,Khera, Pawan,Varshney, Rajeev K.,Pandey, Manish K.,Qiao, Lixian,Feng, Suping,Khera, Pawan,Wang, Hui,Culbreath, Albert K.,Guo, Baozhu,Wang, Ming Li,Tonnis, Brandon,Barkley, Noelle A.,Qiao, Lixian,Feng, Suping,Wang, Hui,Wang, Jianping,Holbrook, C. Corley. 2014

[16]Transcriptomic and Physiological Evidence for the Relationship between Unsaturated Fatty Acid and Salt Stress in Peanut. Sui, Na,Wang, Yu,Liu, Shanshan,Yang, Zhen,Wang, Fang,Wan, Shubo. 2018

[17]Comparison of the Delta(12) fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes. Yu, Shanlin,Pan, Lijuan,Zhang, Hongsheng,Pan, Lijuan,Yang, Qingli,Min, Ping,Ren, Zengkai. 2008

[18]Transcriptome identification of the resistance-associated genes (RAGs) to Aspergillus flavus infection in pre-harvested peanut (Arachis hypogaea). Chen, Xiao-Ping,Li, Hai-Fen,Liu, Hai-Yan,Hong, Yan-Bin,Liang, Xuan-Qiang,Wang, Tong,Li, Ling,Liang, Xuan-Qiang,Wang, Tong,Yang, Qing-Li,Chi, Xiao-Yuan,Yang, Zhen,Yu, Shan-Lin. 2013

[19]Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG(1) from peanut (Arachis hypogaea L.). Wan, Shu-bo,Shan, Shi-hua,Zhang, Ting-ting,Li, Chun-juan,Yang, Chen,Yan, Cai-xia,Yang, Chen. 2011

[20]Cloning and characterization of SPL-family genes in the peanut (Arachis hypogaea L.). Li, M.,Zhao, S. Z.,Zhao, C. Z.,Zhang, Y.,Xia, H.,Wan, S. B.,Wang, X. J.,Lopez-Baltazar, J.. 2016

作者其他论文更多>>

Comparative Transcriptome Analysis of Two Contrasting Chinese Cabbage (Brassica rapa L.) Genotypes Reveals that Some Kinds of Phytohormones Accumulation Involved in the Local Bulges Formation on the Leaf Surface

作者：Zhang, Y.;Zhang, S.;Wang, L.;Zhang, H.;Wang, F.;Gao, J.;Zhang, Y.;Zhang, H.

关键词：Brasscia rapa; crinkly; phytohormones; gene expression
Physiological and molecular responses of two Chinese cabbage genotypes to heat stress

作者：Song, Q.;Yang, F.;Cui, B.;Li, J.;Zhang, Y.;Li, H.;Wang, F.;Gao, J.;Song, Q.;Yang, F.;Cui, B.;Li, J.;Zhang, Y.;Li, H.;Wang, F.;Gao, J.;Song, Q.;Yang, F.;Cui, B.;Li, J.;Zhang, Y.;Li, H.;Wang, F.;Gao, J.;Song, Q.;Cui, B.;Qiu, N.;Yang, F.;Qiu, N.

关键词：antioxidants; chlorophyll fluorescence; heat shock; malondialdehyde; proline; proteins; sugars; transcription factor
Physiological and molecular responses of two Chinese cabbage genotypes to heat stress

作者：Song, Q.;Yang, F.;Cui, B.;Li, J.;Zhang, Y.;Li, H.;Wang, F.;Gao, J.;Song, Q.;Yang, F.;Cui, B.;Li, J.;Zhang, Y.;Li, H.;Wang, F.;Gao, J.;Song, Q.;Yang, F.;Cui, B.;Li, J.;Zhang, Y.;Li, H.;Wang, F.;Gao, J.;Song, Q.;Cui, B.;Qiu, N.;Yang, F.;Qiu, N.

关键词：antioxidants; chlorophyll fluorescence; heat shock; malondialdehyde; proline; proteins; sugars; transcription factor
Genome-Wide Gene Expression Profiles of Orange and White Leafy Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) during the Summer Production Season

作者：Zhang, Y.;Li, X.;Ding, Q.;Li, J. J.;Wang, F. D.;Gao, J. W.;Zhang, Y.;Li, X.;Ding, Q.;Li, J. J.;Wang, F. D.;Gao, J. W.;Zhang, Y.;Li, X.;Ding, Q.;Li, J. J.;Wang, F. D.;Gao, J. W.;Li, X.;Gao, J. W.

关键词：Brassica rapa; Chinese cabbage; orange head; heat stress; RNA-seq
Global transcriptome analysis provides new insights in Thellungiella salsuginea stress response

作者：Zhang, Y.;Shi, S. H.;Li, F. L.;Zhao, C. Z.;Li, A. Q.;Hou, L.;Xia, H.;Wang, X. J.;Zhao, S. Z.;Shi, S. H.;Zhao, C. Z.;Xia, H.;Wang, B. S.;Wang, X. J.;Zhao, S. Z.;Li, F. L.;Baltazar, J. L.

关键词：Exogenous ABA; gene expression; RNA-seq; Thellungiella salsuginea
Genome-Wide Gene Expression Profiles of Orange and White Leafy Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) during the Summer Production Season

作者：Zhang, Y.;Li, X.;Ding, Q.;Li, J. J.;Wang, F. D.;Gao, J. W.;Zhang, Y.;Li, X.;Ding, Q.;Li, J. J.;Wang, F. D.;Gao, J. W.;Zhang, Y.;Li, X.;Ding, Q.;Li, J. J.;Wang, F. D.;Gao, J. W.;Li, X.;Gao, J. W.

关键词：Brassica rapa; Chinese cabbage; orange head; heat stress; RNA-seq
Global transcriptome analysis provides new insights in Thellungiella salsuginea stress response

作者：Zhang, Y.;Shi, S. H.;Li, F. L.;Zhao, C. Z.;Li, A. Q.;Hou, L.;Xia, H.;Wang, X. J.;Zhao, S. Z.;Shi, S. H.;Zhao, C. Z.;Xia, H.;Wang, B. S.;Wang, X. J.;Zhao, S. Z.;Li, F. L.;Baltazar, J. L.

关键词：Exogenous ABA; gene expression; RNA-seq; Thellungiella salsuginea

Cloning of Acyl-ACP thioesterase FatA from Arachis hypogaea L. and its expression in Escherichia coli

作者其他论文 更多>>

Comparative Transcriptome Analysis of Two Contrasting Chinese Cabbage (Brassica rapa L.) Genotypes Reveals that Some Kinds of Phytohormones Accumulation Involved in the Local Bulges Formation on the Leaf Surface

Physiological and molecular responses of two Chinese cabbage genotypes to heat stress

Physiological and molecular responses of two Chinese cabbage genotypes to heat stress

Genome-Wide Gene Expression Profiles of Orange and White Leafy Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) during the Summer Production Season

Global transcriptome analysis provides new insights in Thellungiella salsuginea stress response

Genome-Wide Gene Expression Profiles of Orange and White Leafy Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) during the Summer Production Season

Global transcriptome analysis provides new insights in Thellungiella salsuginea stress response

意 见 箱

作者其他论文更多>>

意见箱