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Effect of BO-ACO 2 gene on post-harvest senescence in transgenic broccoli (Brassica oleracea L. var. italica)

文献类型： 外文期刊

作者： Qin, Feifei 1 ; Wang, Chengrong 1 ; Wang, Ran 1 ; Ma, Gang 1 ; Xu, Hui-lian 3 ;

作者机构： 1.Qingdao Agr Univ, Coll Hort, Qingdao 266109, Peoples R China

2.Shandong Acad Agr Sci, Peanut Res Inst, Qingdao 266100, Peoples R China

3.Int Nat Farming Res Ctr, Nagano 3901401, Japan

关键词： ACC oxidase;BO-ACO 2 gene;ethylene;real-time PCR;senescence;transgenic broccoli

期刊名称：JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT （ 影响因子：0.435； 五年影响因子：0.484 ）

ISSN： 1459-0255

年卷期： 2011 年 9 卷 2 期

页码：

收录情况： SCI

摘要： Transgenic broccoli plants carrying an antisense (AS) or a sense (S) BO-ACO 2 gene were successfully obtained by Agrobacterium tumefaciens-mediated method in our previous study. In the present study, the effects of BO-ACO 2 gene on ethylene biosynthesis and post-harvest senescence of transgenic broccoli were analyzed. Transgenic broccoli showed significantly extended shelf life through delaying onset of yellowing by approximately 3 days in florets of the AS line transgenic broccoli plants and by approximately 12 hours in florets of the S line plants. This result was confirmed by related physiological analyses on chlorophyll level, floral respiration rate, ethylene production and 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase activity. The chlorophyll content in control broccoli decreased rapidly after harvest, while transgenic broccoli showed a low decreased rate of chlorophyll loss and an improvement in head color. Florets from the AS line produced significantly less post-harvest ethylene over the 156 h experimental period, and no obvious peak was observed. Patterns of the ethylene production in florets of the S line were similar to those in control, but the maximum was significantly reduced and delayed 12 hours. ACC oxidase activity in the AS line maintained at a very low level during the whole experimental period. The curve of ACC oxidase activity in the S line was similar to that of the control within 48 hours after harvest; however, the second peak appeared at 60 hour. Real-time Fluorescence PCR analyses showed that the highest expression of BO-ACO 2 gene in the AS line was 36 hours later than that in control due to the antisense BO-ACO 2 gene though its level was close to control. The expression of the BO-ACO 2 gene was largely suppressed during the experimental period in the S line. In conclusion, the shelf life of broccoli can be prolonged 1 day (sense BO-ACO 2) or 3 days (antisense BO-ACO 2) by reducing post-harvest ethylene.

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