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PwHAP5, a CCAAT-binding transcription factor, interacts with PwFKBP12 and plays a role in pollen tube growth orientation in Picea wilsonii

文献类型： 外文期刊

作者： Yu, Yanli 2 ; Li, Yanze 2 ; Huang, Guixue 1 ; Meng, Zhaodong 3 ; Zhang, Dun 1 ; Wei, Jing 1 ; Yan, Kang 2 ; Zheng, Chengc 1 ;

作者机构： 1.Beijing Forestry Univ, Minist Educ, Key Lab Forest Silviculture & Conservat, Beijing 100083, Peoples R China

2.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

3.Shandong Acad Agr Sci, Maize Inst, Natl Maize Improvement Sub Ctr, Jinan 250100, Peoples R China

4.Shandong Acad Agr Sci, Maize Inst, Natl Maize Improvement Sub Ctr, Jinan 250100, Peop

关键词： Growth orientation;Picea wilsonii;pollen tube;PwFKBP12;PwHAP5

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2011 年 62 卷 14 期

页码：

收录情况： SCI

摘要： The HAP complex occurs in many eukaryotic organisms and is involved in multiple physiological processes. Here it was found that in Picea wilsonii, HAP5 (PwHAP5), a putative CCAAT-binding transcription factor gene, is involved in pollen tube development and control of tube orientation. Quantitative real-time reverse transcription-PCR showed that PwHAP5 transcripts were expressed strongly in germinating pollen and could be induced by Ca(2+). Overexpression of PwHAP5 in pollen altered pollen tube orientation, whereas the tube with PwHAP5RNAi showed normal growth without diminishing pollen tube growth. Furthermore, PwFKBP12, which encodes an FK506-binding protein (FKBP) was screened and a bimolecular fluorescence complementation assay performed to confirm the interaction of PwHAP5 and PwFKBP12 in vivo. Transient expression of PwFKBP12 in pollen showed normal pollen tube growth, whereas the tube with PwFKBP12RNAi bent. The phenotype of overexpression of HAP5 on pollen tube was restored by FKBP12. Altogether, our study supported the role of HAP5 in pollen tube development and orientation regulation and identified FKBP12 as a novel partner to interact with HAP5 involved in the process.

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