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A long non-coding RNA functions as a competitive endogenous RNA to modulate TaNAC018 by acting as a decoy for tae-miR6206

文献类型： 外文期刊

作者： Xu, Wei-Bo 1 ; Guo, Qian-Huan 1 ; Liu, Peng 3 ; Dai, Shuang 2 ; Wu, Chang-Ai 1 ; Yang, Guo-Dong 1 ; Huang, Jin-Guang 1 ; Zhang, Shi-Zhong 1 ; Song, Jian-Min 2 ; Zheng, Cheng-Chao 1 ; Yan, Kang 1 ;

作者机构： 1.Shandong Agr Univ, Coll Life Sci, Natl Key Lab Wheat Improvement, Tai An 271018, Shandong, Peoples R China

2.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Shandong, Peoples R China

3.Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA

关键词： Wheat; Drought stress; Cuticular waxes; lncRNA; miRNA; NAC

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：5.1； 五年影响因子：5.3 ）

ISSN： 0167-4412

年卷期： 2024 年 114 卷 3 期

页码：

收录情况： SCI

摘要： Increasing evidence indicates a strong correlation between the deposition of cuticular waxes and drought tolerance. However, the precise regulatory mechanism remains elusive. Here, we conducted a comprehensive transcriptome analysis of two wheat (Triticum aestivum) near-isogenic lines, the glaucous line G-JM38 rich in cuticular waxes and the non-glaucous line NG-JM31. We identified 85,143 protein-coding mRNAs, 4,485 lncRNAs, and 1,130 miRNAs. Using the lncRNA-miRNA-mRNA network and endogenous target mimic (eTM) prediction, we discovered that lncRNA35557 acted as an eTM for the miRNA tae-miR6206, effectively preventing tae-miR6206 from cleaving the NAC transcription factor gene TaNAC018. This lncRNA-miRNA interaction led to higher transcript abundance for TaNAC018 and enhanced drought-stress tolerance. Additionally, treatment with mannitol and abscisic acid (ABA) each influenced the levels of tae-miR6206, lncRNA35557, and TaNAC018 transcript. The ectopic expression of TaNAC018 in Arabidopsis also improved tolerance toward mannitol and ABA treatment, whereas knocking down TaNAC018 transcript levels via virus-induced gene silencing in wheat rendered seedlings more sensitive to mannitol stress. Our results indicate that lncRNA35557 functions as a competing endogenous RNA to modulate TaNAC018 expression by acting as a decoy target for tae-miR6206 in glaucous wheat, suggesting that non-coding RNA has important roles in the regulatory mechanisms responsible for wheat stress tolerance.

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