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Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene

文献类型： 外文期刊

作者： Zhang, Yue 1 ; Xia, Han 2 ; Yuan, Mei 3 ; Zhao, Chuanzhi 2 ; Li, Aiqin 2 ; Wang, Xingjun 1 ;

作者机构： 1.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China

2.Shandong Acad Agr Sci, Hightech Res Ctr, Key Lab Genet Improvement Crop Anim & Poultry Sha, Jinan, Peoples R China

3.Shandong Peanut Res Inst, Qingdao, Shandong, Peoples R China

关键词： Arachis hypogaea L.;cDNA library;chalcone isomerase;expression analysis

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）

ISSN： 0717-3458

年卷期： 2012 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Chalcone isomerase (CHI) is the key enzyme that catalyzes chalcone into (2S)-flavanol or (2S)-5-desoxidation flavanol. The full length cDNA (1050 bp) of AhCHI (Arachis hypogaea CHI gene) was cloned by large scale EST sequencing using a peanut immature seed cDNA library. Sequence analysis results indicated that it was a type I CHI gene (with the accession number JN660794). The ORF of AhCHI was 768 bp, encoding a peptide of 255 amino acids with a pI of 5.189. Sequence alignment showed that the coding region of AhCHI gene is highly conserved to compare with CHI genes from other plant species. Peanut cDNA microarray and semi-quantitative RT-PCR analysis indicated that AhCHI was highly expressed in pegs. The expression level in flower and root was higher than the expression level in stem and leaf. AhCHI was expressed in a high level in seeds with a purple seed coat, while its expression was low in seed with white seed coat.

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