Identification of New Delhi Metallo-beta-lactamase 1 in Acinetobacter Iwoffii of Food Animal Origin
文献类型： 外文期刊
作者： Wang, Yang 1 ; Wu, Congming 1 ; Zhang, Qijing 2 ; Qi, Jing; Liu, Hebing 1 ; Wang, Yu 1 ; He, Tao 1 ; Ma, Licai 1 ; Lai, J 1 ;
作者机构： 1.China Agr Univ, Key Lab Dev & Evaluat Chem & Herbal Drugs Anim Us, Coll Vet Med, Beijing 100094, Peoples R China
2.Iowa State Univ, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA USA
3.Iowa State Univ, Coll Vet Med, De
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2012 年 7 卷 5 期
页码：
收录情况： SCI
摘要： Background: To investigate the presence of metallo-beta-lactamase (MBL) genes and the genetic environment of the New Delhi metallo-beta-lactamase gene bla(NDM-1) in bacteria of food animal origin. Methodology/Principal Findings: Gram-negative bacteria with low susceptibility to imipenem (MIC>8 mu g/mL) were isolated from swab samples collected from 15 animal farms and one slaughterhouse in eastern China. These bacteria were selected for phenotypic and molecular detection of known MBL genes and antimicrobial susceptibility testing. For the bla(NDM-1) positive isolate, conjugation and transformation experiments were carried out to assess plasmid transfer. Southern blotting was conducted to localize the bla(NDM-1) genes, and DNA sequencing was performed to determine the sequences of bla(NDM-1) and the flanking genes. In total, nine Gram-negative bacteria of four different species presented a MBL phenotype. bla(NDM-1) was identified on a mobile plasmid named pAL-01 in an Acinetobacter Iwoffii isolate of chicken origin. Transfer of pAL-01 from this isolate to E. coli J53 and JM109 resulted in resistance to multiple beta-lactams. Sequence analysis revealed that the bla(NDM-1) gene is attached to an intact insertion element ISAba125, whose right inverted repeat (IR-R) overlaps with the promoter sequence of bla(NDM-1). Thus, insertion of ISAba125 likely enhances the expression of bla(NDM-1). Conclusion: The identification of a bla(NDM-1)-carrying strain of A. Iwoffii in chickens suggests the potential for zoonotic transmission of bla(NDM-1) and has important implications for food safety.
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