您好，欢迎访问山东省农业科学院文献资源数据库平台

Short communication: A mutation in the 3 ' untranslated region diminishes microRNA binding and alters expression of the OLR1 gene

文献类型： 外文期刊

作者： Wang, X. 1 ; Li, T. 1 ; Zhao, H. B. 2 ; Khatib, H. 3 ;

作者机构： 1.Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China

2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Shandong, Peoples R China

3.Univ Wisconsin, Dept Anim Sci, Madison, WI 53706 USA

关键词： oxidized low-density lipoprotein receptor 1 (OLR1) gene;Bos taurus autosome microRNA 370 (bta-mir-370);single nucleotide polymorphism

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2013 年 96 卷 10 期

页码：

收录情况： SCI

摘要： The oxidized low-density lipoprotein (lectin-like) receptor 1 (OLR1) gene plays an important role in the degradation of the oxidized low-density lipoprotein, which causes damage to the arterial endothelium. Previous studies have shown that a single nucleotide polymorphism (SNP) in the 3' untranslated region (UTR) of OLR1 was associated with milk production and health traits in dairy cattle and with loin eye area and marbling depth in Qinchuan beef cattle. However, the mechanisms by which this SNP affects these traits are not well understood. MicroRNA (miRNA or mir) are small noncoding RNA that regulate gene expression by binding to target mRNA at their UTR to degrade or to repress translation of the target transcript. We hypothesized that miRNA bind to the 3' UTR of OLR1 to cause expression changes of the gene. To test this hypothesis, the Bos taurus autosome (bta)-mir-370 miRNA was selected for this study based on bioinformatics prediction analysis. Two vectors that included A or C nucleotides of the 3' UTR SNP and 1 control vector were co-transfected with the vector of bta-miR-370 into human embryonic kidney 293 (HEK293) cells. Results of the dual-luciferase reporter assay showed that the activity of luciferase was significantly lower in cells transfected with the A nucleotide vector than that of the C nucleotide and control vectors. The assay also indicated that activity of miRNA bta-mir-370 was associated with a differential allelic regulation of OLR1 expression. These results imply that the 3' UTR SNP of the OLR1 gene is a strong candidate marker for selection in cattle breeding programs.

  • 相关文献

[1]Single nucleotide polymorphisms, haplotypes and combined genotypes of LAP3 gene in bovine and their association with milk production traits. Zheng, Xue,Ju, Zhihua,Wang, Ji,Li, Qiuling,Huang, Jinming,Zhong, Jifeng,Wang, Changfa,Zheng, Xue,Zhang, Aiwu. 2011

[2]A SNP in the 3 '-UTR of HSF1 in dairy cattle affects binding of target bta-miR-484. Li, Q. L.,Xia, P.,Wang, Y. J.,Wu, Z. Y.,Jia, Y. H.,Chang, S. M.,Zhang, Z. F.,Li, Q. L.,Chu, M. X.. 2015

[3]Identification of Splice Variants, Targeted MicroRNAs and Functional Single Nucleotide Polymorphisms of the BOLA-DQA2 Gene in Dairy Cattle. Hou, Qinlei,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Li, Liming,Wang, Changfa,Sun, Tao,Wang, Lingling,Hou, Minghai,Zhong, Jifeng,Hang, Suqin.

[4]Genome-wide association study for soybean cyst nematode resistance in Chinese elite soybean cultivars. Zhang, Jun,Li, Wei,Zhang, Yanwei,Zhang, Lifeng,Dai, Haiying,Xu, Ran,Wen, Zixiang,Wang, Dechun,Zhang, Jun.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台