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The regulation of PCR by thiolated single-stranded DNA for enhanced specificity

文献类型: 外文期刊

作者: Zhang, Guangyuan 1 ; Sun, Hongwei 1 ; Li, Fan 1 ; Yang, Shuke 1 ; Gao, Rui 1 ; Xu, Xiaohui 1 ; Lu, Xingbo 1 ;

作者机构: 1.Shandong Acad Agr Sci, Inst Plant Protect, Shandong Key Lab Plant Virol, Jinan, Peoples R China

期刊名称:RSC ADVANCES ( 影响因子:3.361; 五年影响因子:3.39 )

ISSN: 2046-2069

年卷期: 2014 年 4 卷 65 期

页码:

收录情况: SCI

摘要: In this study, we observed that 5'-end 6-mercapto-1-hexane-labeled single-stranded DNA (HS-ssDNA) dramatically enhanced the specificity of DNA duplication during the polymerase chain reaction (PCR). The regulation of PCR by HS-ssDNA is both intriguing and useful, and such findings further our understanding of the biochemical characteristics and potential applications of HS-ssDNA.

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