Characterization and Expression of Genes Encoding Three Small Heat Shock Proteins in Sesamia inferens (Lepidoptera: Noctuidae)
文献类型： 外文期刊
作者： Sun, Meng 1 ; Lu, Ming-Xing 1 ; Tang, Xiao-Tian 1 ; Du, Yu-Zhou 1 ;
作者机构： 1.Yangzhou Univ, Sch Hort & Plant Protect, Yangzhou 225009, Peoples R China
2.Yangzhou Univ, Inst Appl Entomol, Yangzhou 225009, Peoples R China
3.Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Peoples R China
关键词： Sesamia inferens;small heat shock protein;cloning;expression pattern
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）
ISSN： 1422-0067
年卷期： 2014 年 15 卷 12 期
页码：
收录情况： SCI
摘要： The pink stem borer, Sesamia inferens (Walker), is a major pest of rice and is endemic in China and other parts of Asia. Small heat shock proteins (sHSPs) encompass a diverse, widespread class of stress proteins that have not been characterized in S. inferens. In the present study, we isolated and characterized three S. inferens genes that encode members of the a-crystallin/sHSP family, namely, Sihsp21.4, Sihsp20.6, and Sihsp19.6. The three cDNAs encoded proteins of 187, 183 and 174 amino acids with calculated molecular weights of 21.4, 20.6 and 19.6 kDa, respectively. The deduced amino acid sequences of the three genes showed strong similarity to sHSPs identified in other lepidopteran insects. Sihsp21.4 contained an intron, but Sihsp20.6 and Sihsp19.6 lacked introns. Real-time quantitative PCR analyses revealed that Sihsp21.4 was most strongly expressed in S. inferens heads; Whereas expression of Sihsp20.6 and Sihsp19.6 was highest in eggs. The three S. inferens sHSP genes were up-regulated during low temperature stress. In summary, our results show that S. inferens sHSP genes have distinct regulatory roles in the physiology of S. inferens.
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