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Transcriptomic Analysis of the Porcine Endometrium during Embryo Implantation

文献类型: 外文期刊

作者: Lin, Haichao 1 ; Wang, Huaizhong 1 ; Wang, Yanping 1 ; Liu, Chang 1 ; Wang, Cheng 1 ; Guo, Jianfeng 1 ;

作者机构: 1.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan 250100, Peoples R China

2.Key Lab Dis Control & Anim Breeding Shandong Prov, Jinan 250100, Peoples R China

关键词: embryo implantation;endometrium;pigs;pregnancy;sows;transcriptome

期刊名称:GENES ( 影响因子:4.096; 五年影响因子:4.339 )

ISSN: 2073-4425

年卷期: 2015 年 6 卷 4 期

页码:

收录情况: SCI

摘要: In pigs, successful embryo implantation is an important guarantee for producing litter size, and early embryonic loss occurring on day 12-30 of gestation critically affects the potential litter size. The implantation process is regulated by the expression of numerous genes, so comprehensive analysis of the endometrium is necessary. In this study, RNA sequencing (RNA-Seq) technology is used to analyze endometrial tissues during early pregnancy. We investigated the changes of gene expression between three stages (day 12, 18, and 25) by multiple comparisons. There were 1557, 8951, and 2345 differentially expressed genes (DEGs) revealed between the different periods of implantation. We selected several genes for validation by the use of quantitative real-time RT-PCR. Bioinformatic analysis of differentially expressed genes in the endometrium revealed a number of biological processes and pathways potentially involved in embryo implantation in the pig, most noticeably cell proliferation, regulation of immune response, interaction of cytokine-cytokine receptors, and cell adhesion. These results showed that specific gene expression patterns reflect the different functions of the endometrium in three stages (maternal recognition, conceptus attachment, and embryo implantation). This study identified comprehensive transcriptomic profile in the porcine endometrium and thus could be a foundation for targeted studies of genes and pathways potentially involved in abnormal endometrial receptivity and embryo loss in early pregnancy.

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