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-catenin promotes intracellular bacterial killing via suppression of Pseudomonas aeruginosa-triggered macrophage autophagy

文献类型： 外文期刊

作者： Fu, Qiang 1 ; Chen, Kang 1 ; Zhu, Qian 2 ; Wang, Weijia 1 ; Huang, Fuda 1 ; Miao, Lishao 1 ; Wu, Xinger 1 ;

作者机构： 1.Sun Yat Sen Univ, Zhongshan Hosp, Div Clin Lab, 2 Sunwendong Rd, Zhongshan 528403, Guangdong, Peoples R China

2.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan, Peoples R China

关键词： Pseudomonas aeruginosa;-catenin;bacterial killing;autophagy

期刊名称：JOURNAL OF INTERNATIONAL MEDICAL RESEARCH （ 影响因子：1.671； 五年影响因子：1.805 ）

ISSN： 0300-0605

年卷期： 2017 年 45 卷 2 期

页码：

收录情况： SCI

摘要： Objective To investigate -catenin-mediated bacterial elimination during Pseudomonas aeruginosa infection of macrophage-like RAW264.7 cells. Methods Cell viability and catenin beta 1 (CTNNB1) expression in RAW264.7 cells following P. aeruginosa infection versus uninfected cells, were detected by cell counting kit-8 assay and -catenin Western blots. RAW264.7 cells with CTNNB1 overexpression were established with -catenin lentivirus using flow cytometry and clonogenic limiting dilution assays. Bacterial killing was measured by plate counts; phagocytosis and nitric oxide (NO) were measured by flow cytometry; and reactive oxygen species (ROS) were measured using Griess reaction. Autophagy was determined by microtubule-associated protein 1 light chain 3 alpha-phosphatidylethanolamine conjugate (LC3-II) protein levels and formation of LC3 puncta, using Western blot and immunofluorescence staining. Results Following P. aeruginosa infection, RAW264.7 cell -catenin levels were reduced in a time- and multiplicity of infection-dependent manner. CTNNB1 overexpression was associated with increased P. aeruginosa elimination, but had no effect on RAW264.7 cell phagocytosis, ROS and NO. CTNNB1 overexpression reduced LC3-II levels and formation of LC3 puncta, suggesting autophagy inhibition. Rapamycin/starvation-induced autophagy resulted in reduced bacterial killing following P. aeruginosa infection. Conclusion -catenin may promote bacterial killing via suppression of P. aeruginosa-induced macrophage autophagy.

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