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Identification of a peptide derived from the heptad repeat 2 region of the porcine epidemic diarrhea virus (PEDV) spike glycoprotein that is capable of suppressing PEDV entry and inducing neutralizing antibodies

文献类型： 外文期刊

作者： Zhao, Pengwei 1 ; Wang, Bin 2 ; Ji, Chun-Miao 2 ; Cong, Xiaoyan; Wang, Ming 1 ; Huang, Yao-Wei 2 ;

作者机构： 1.China Agr Univ, Coll Vet Med, Key Lab Zoonosis, Minist Agr, 2 Yuan Ming Yuan,West Rd, Beijing 100193, Peoples R China

2.Zhejiang Univ, Coll Anim Sci, Inst Prevent Vet Med, Hangzhou 310058, Zhejiang, Peoples R China

3.Zhejiang Univ, Coll Anim Sci, Key Lab Anim Virol, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China

4.Zhejiang Univ, Coll Anim Sci, Key Lab Anim Virol, Minist Agr, Hangzhou 310058, Zheji

关键词： Porcine epidemic diarrhea virus (PEDV);Heptad repeat (HR) regions;Viral entry;Spike glycoprotein;Neutralizing antibody

期刊名称：ANTIVIRAL RESEARCH （ 影响因子：5.97； 五年影响因子：5.801 ）

ISSN： 0166-3542

年卷期： 2018 年 150 卷

页码：

收录情况： SCI

摘要： Heptad repeat (HR) regions are highly conserved motifs located in the glycoproteins of enveloped viruses that form a six-helix bundle structure and is important in the process of virus fusion. Peptides derived from the HR regions of some viruses have also been shown to inhibit viral entry. Porcine epidemic diarrhea virus (PEDV) was predicted to have HR regions (HR1 and HR2) in the spike glycoprotein S2 subunit. Based on this analysis, six peptides derived from HR1 and HR2 were selected, expressed in Escherichia coli, purified, and characterized. Three peptides (HR2M, HR2L and HR2P) were identified as potential competitive inhibitors in PEDV in vitro infection assays, with the HR2P peptide representing the most potent inhibitor. Further study indicated that immunization of HR2P in mice elicited antibodies capable of neutralizing PEDV infection in vitro. These results demonstrate that the HR2P peptide and anti-HR2P antibody can serve as a tool for dissecting the fusion mechanism of PEDV, guiding the search for potent inhibitors with therapeutic value against PEDV infection.

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[1]Molecular Characterization and Phylogenetic Analysis of New Variants of the Porcine Epidemic Diarrhea Virus in Gansu, China in 2012. Tian, Yufei,Liu, Yuxiu,Yang, Zhenguo,Tian, Yufei,Yu, Zhijun,Huang, Jing,Xin, Yue,Li, Yuanguo,Wang, Tiecheng,Huang, Geng,Feng, Na,Yang, Songtao,Gao, Yuwei,Xia, Xianzhu,Yu, Zhijun,Fan, Shengtao,Yu, Zhijun,Fan, Shengtao,Cheng, Kaihui. 2013

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