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Genome-wide characterization of differentially expressed genes provides insights into regulatory network of heat stress response in radish (Raphanus sativus L.)

文献类型： 外文期刊

作者： Wang, Ronghua 1 ; Mei, Yi 3 ; Xu, Liang 1 ; Zhu, Xianwen 4 ; Wang, Yan 1 ; Guo, Jun 3 ; Liu, Liwang 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Hort, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

2.Shandong Acad Agr Sci, Inst Vegetables & Flowers, Jinan 250100, Shandong, Peoples R China

3.Yancheng Acad Agr Sci, Yancheng 224002, Jiangsu, Peoples R China

4.North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA

关键词： Heat stress;Raphanus sativus L.;Differentially expressed gene (DEG);RNA-Seq;Regulatory network

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

ISSN： 1438-793X

年卷期： 2018 年 18 卷 2 期

页码：

收录情况： SCI

摘要： Heat stress (HS) causes detrimental effects on plant morphology, physiology, and biochemistry that lead to drastic reduction in plant biomass production and economic yield worldwide. To date, little is known about HS-responsive genes involved in thermotolerance mechanism in radish. In this study, a total of 6600 differentially expressed genes (DEGs) from the control and Heat24 cDNA libraries of radish were isolated by high-throughput sequencing. With Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, some genes including MAPK, DREB, ERF, AP2, GST, Hsf, and Hsp were predominantly assigned in signal transductions, metabolic pathways, and biosynthesis and abiotic stress-responsive pathways. These pathways played significant roles in reducing stress-induced damages and enhancing heat tolerance in radish. Expression patterns of 24 candidate genes were validated by reverse-transcription quantitative PCR (RT-qPCR). Based mainly on the analysis of DEGs combining with the previous miRNAs analysis, the schematic model of HS-responsive regulatory network was proposed. To counter the effects of HS, a rapid response of the plasma membrane leads to the opening of specific calcium channels and cytoskeletal reorganization, after which HS-responsive genes are activated to repair damaged proteins and ultimately facilitate further enhancement of thermotolerance in radish. These results could provide fundamental insight into the regulatory network underlying heat tolerance in radish and facilitate further genetic manipulation of thermotolerance in root vegetable crops.

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