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Phylogenetic analysis and rapid identification of the whitefly, Bemisia afer, in China

文献类型： 外文期刊

作者： Chu, Dong 1 ; Liu, Guoxia 1 ; Wan, Fanghao 2 ; Tao, Yunli 1 ; Gill, Ray J. 3 ;

作者机构： 1.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan 250100, Shandong, Peoples R China

2.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect S Campus, Beijing 100081, Peoples R China

3.Plant Pest Diagnost Ctr, Sacramento, CA 95832 USA

关键词： mitochondrial cytochrome oxidase I; molecular markers

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）

ISSN：

年卷期： 2010 年 10 卷

页码：

收录情况： SCI

摘要： The phylogenetic relationship between the whitefly Bemisia afer (Priesner & Hosny) (Hemiptera: Aleyrodidae) from China and other populations among the world were analyzed based on the mitochondrial cytochrome oxidase I (mtCOI) gene. Phylogenetic analysis of mtCOI sequences and those of reference B. afer sequences showed that the populations of the species could be separated into 5 clades (I-V). There were at least two clades of the species from China (IV and V). These data suggested that B. afer might be a species complex. The Chinese B. afer populations were most divergent with B. afer from the United Kingdom and African countries. The distance between the Chinese B. afer (IV and V) and clades I, II, and III is more than 32%, while the distance among clades I, II, III is lower than 7.7%. A new set of primers specific to B. afer was designed to amplify a region of approximately 400 bp to discriminate B. afer from other Bemisia species in China based on mtCOI sequences.

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