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Identification of a Pm4 Allele Conferring Powdery Mildew Resistance in Wheat Breeding Line GR18-1

文献类型： 外文期刊

作者： Su, Fuyu 1 ; Han, Guohao 2 ; Yu, Ziyang 1 ; Liu, Cheng 3 ; Li, Haosheng 3 ; Wang, Xiaolu 3 ; Chang, Lin 4 ; Xiao, Luning 1 ; Mu, Yanjun 1 ; Bian, Qingzhong 5 ; Wang, Fengtao 6 ; Jin, Yuli 1 ; Ma, Pengtao 1 ;

作者机构： 1.Yantai Univ, Sch Life Sci, Yantai 264005, Peoples R China

2.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Agr Resources Res, Shijiazhuang 050021, Peoples R China

3.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Peoples R China

4.Yantai Fushan Agr Bur, Yantai 265599, Peoples R China

5.Jiangsu Qianchonglang Agr Technol Co Ltd, Gaoyou 225600, Peoples R China

6.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

关键词： Blumeria graminis f; sp; tritici; MAS; Pm4; Triticum aestivum L; wheat breeding line

期刊名称：PLANT DISEASE （ 影响因子：4.5； 五年影响因子：5.0 ）

ISSN： 0191-2917

年卷期： 2023 年 107 卷 7 期

页码：

收录情况： SCI

摘要： Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is a serious fungal wheat disease of wheat worldwide. Host resistance is considered to be the most environmentally friendly and efficient approach against this disease. Wheat breeding line GR18-1 showed resistance to powdery mildew at both seedling and adult stages for several years. Genetic analysis indicated that a single dominant gene, tentatively designated as PmGR-18, conferred powdery mildew resistance in GR18-1. Bulked segregant analysis and marker analysis showed that PmGR-18 was located in the Pm4 interval on chromosome arm 2AL and was flanked by the markers Xwgrc763 and Xwgrc872, respectively, with genetic distances of 0.5 and 1.0 cM corresponding to a physical interval of 1.13 Mb based on the Chinese Spring reference genome sequence v2.1. Using homology-based cloning and Sanger sequencing, we found that the sequence of PmGR-18 was totally consistent with that of Pm4d. qRT-PCR analysis showed that the expression levels of two splicing variants Pm4d_V1 and Pm4d_V2 in GR18-1 were significantly upregulated after inoculating with Bgt isolate E09, and the level of Pm4d_V2 was significantly lower than that of Pm4d_V1 at most of the time points, suggesting a different resistance pattern may be involved in the genotype. To facilitate the transfer of PmGR-18 in marker-assisted selection (MAS) breeding, the flanked markers Xwgrc763 and Xwgrc872 and the functional marker JS717/JS718 were tested and confirmed to enable the tracking of PmGR-18 when it transferred into those susceptible cultivars.

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