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Interference-resistant aptasensor with tetrahedral DNA nanostructure for profenofos detection based on the composites of graphene oxide and polyaniline

文献类型： 外文期刊

作者： Wang, Guanjie 1 ; Dong, Haowei 1 ; Han, Jie 1 ; Zhang, Mei 1 ; Huang, Jingcheng 1 ; Sun, Jiashuai 1 ; Guan, Fukai 1 ; Shen, Zhen 1 ; Xu, Deyan 1 ; Sun, Xia 1 ; Guo, Yemin 1 ; Zhao, Shancang 1 ;

作者机构： 1.Shandong Univ Technol, Sch Agr Engn & Food Sci, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China

2.Shandong Prov Engn Res Ctr Vegetable Safety & Qual, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China

3.Zibo City Key Lab Agr Prod Safety Traceabil, 266 Xincun Xilu, Zibo 255049, Shandong, Peoples R China

4.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan 250100, Shandong, Peoples R China

关键词： Electrochemical aptasensor; Gold nanoparticles-tetrahedral DNA nano-structure; Graphene oxide and polyaniline; Profenofos

期刊名称：BIOELECTROCHEMISTRY （ 影响因子：5.76； 五年影响因子：5.368 ）

ISSN： 1567-5394

年卷期： 2022 年 148 卷

页码：

收录情况： SCI

摘要： In this work, an interference-resistant electrochemical aptasensor that could detect profenofos in vegetables was constructed based on complexes of graphene oxide and polyaniline (GO@PANI) and gold nanoparticles-tetrahedral DNA nanostructure (Au-TDN). Compared with a single chain aptamer, the tetrahedral DNA nano -structure is highly stable and allows the aptamer on this structure to stand in a highly ordered position on an electrode surface. Moreover, the AuNPs are biocompatible and can protect the activity of the aptamer, which can improve the assembly success rate of Au-TDN. Besides, the conductivity of PANI had been tremendously enhanced thanks to the existence of GO, which improved the dispersion of PANI. The GO@PANI was prepared by a chemical synthesis method, which had a large surface area and was able to adsorb many Au-TDN. Under optimal working parameters, the constructed aptasensor exhibited good electrochemical sensing performance with a detection limit of 10.50 pg/mL and a linear range of 1.0 x 10(2)-1.0 x 10(7) pg/mL. In addition, it was employed in detecting profenofos in vegetables with a good recovery rate of 90.41-116.37 %. More importantly, the aptasensor also has excellent stability and high selectivity. This study provides a promising method to avoid interference in the detection of profenofos by sensors.

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