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Association of the expression of Th cytokines with peripheral CD4 and CD8 lymphocyte subsets after vaccination with FMD vaccine in Holstein young sires

文献类型： 外文期刊

作者： Yang, Ling 1 ; Liu, Zhichao 1 ; Li, Jianbin 2 ; He, Kaili 1 ; Kong, Lingna 2 ; Guo, Runqing 1 ; Liu, Wenjiao 3 ; Gao, Yundong 2 ; Zhong, Jifeng 2 ;

作者机构： 1.Hebei Univ Engn, Coll Life Sci & Food Engn, Handan 056021, Peoples R China

2.Shandong Acad Agr Sci, Dairy Cattle Res Ctr, Jinan 250100, Shandong, Peoples R China

3.Dairy Assoc China, Beijing 100193, Peoples R China

关键词： Foot-and-mouth disease; Interferon-gamma; Peripheral blood lymphocyte; Lymphocyte subset; Young sire

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN： 0034-5288

年卷期： 2018 年 119 卷

页码：

收录情况： SCI

摘要： High immune response (HIR) cows have a balanced and robust host defense and lower disease incidence, and immune response is more important to consider for selecting young sires than for selecting cows. The protective immune response against foot-and-mouth disease (FMD) virus infection is T-cell-independent in an animal experimental model. However, there is no convenient method to select young sires with a HIR to FMD virus. In this study, 39 healthy Holstein young sires were vaccinated with the trivalent (A, O and Asia 1) FMD vaccine, and T-lymphocyte subsets in peripheral blood lymphocytes (PBLs) were detected using flow cytometric analysis before and after vaccination. The expression of interferon-gamma (IFN-gamma), interleukin-2 (IL-2), IL-4, and IL-6 mRNA in PBLs was analyzed after stimulation by lipopolysaccharide (LPS) or Concanavalin A (ConA) after vaccination. According to the percentage of CD4(+) lymphocyte and CD4/CD8 ratio after vaccination for selecting the HIR young sires, the results showed that the percentages of CD3(+), CD4(+), CD3(+) CD4(+) lymphocytes and the CD4/CD8 ratio in the HIR group were higher compared to those in the medium immune response (MIR) and low immune response (LIR) groups before vaccination. Additionally, the percentage of CD4(+) lymphocytes and the CD4/CD8 ratio after vaccination were positively associated with the expression level of IFN-gamma mRNA in the PBLs after stimulation by LPS. In conclusion, the in vitro expression level of IFN-gamma mRNA in the PBLs stimulated by LPS may serve as a parameter for selecting young sires with a HIR to FMD virus.

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[1]Association of the expression of Th cytokines with peripheral CD4 and CD8 lymphocyte subsets after vaccination with FMD vaccine in Holstein young sires. Yang, Ling,Liu, Zhichao,He, Kaili,Guo, Runqing,Li, Jianbin,Kong, Lingna,Gao, Yundong,Zhong, Jifeng,Liu, Wenjiao. 2018

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