An Antennae-Enriched Odorant-Binding Protein EonuOBP43 Mediate the Behavioral Response of the Tea Green Leafhopper, Empoasca onukii Matsuda to the Host and Nonhost Volatiles
文献类型： 外文期刊
作者： Lun, Xiaoyue 1 ; Xu, Xiuxiu 2 ; Zhang, Yu 1 ; Zhang, Ruirui 1 ; Cao, Yan 1 ; Zhang, Xiangzhi 1 ; Jin, Meina 1 ; Zhang, Zhengqun 1 ; Zhao, Yunhe 1 ;
作者机构： 1.Shandong Agr Univ, Tai An 271018, Peoples R China
2.Shandong Acad Agr Sci, Tea Res Inst, Jinan 250100, Peoples R China
关键词： Empoasca onukii Matsuda; plant volatiles; fluorescence competitivebinding assay; site-directedmutagenesis; RNA interference; behavioral response
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2023 年 71 卷 50 期
页码：
收录情况： SCI
摘要： Olfaction is crucial for Empoasca onukii Matsuda to recognize odors from the host and nonhost plants, and it has been proposed that odorant binding proteins are directly required for odorant discrimination and represent potential targets of interest for pest control. Here, we cloned EonuOBP43 and expressed the recombinant EonuOBP43 protein. Furthermore, competitive fluorescence binding assays with 19 ligands indicated that terpenoids and alkanes showed a relatively higher than for other classes of chemicals. Additionally, ligand docking and site-directed mutagenesis results revealed that seven hydrophobic residues, including Val-86, Met-89, Phe-90, Ile-104, Ile-105, Leu-130, and Val-134, played a key role in the binding of EonuOBP43 to plant volatiles. In olfactometer tests, E. onukii were significantly attracted to alpha-farnesene and repelled to beta-caryophyllene, and dsOBP43 treated adult lost response to alpha-farnesene and beta-caryophyllene. In summary, our results demonstrated that EonuOBP43 may function as a carrier in the process of sensing plant compounds of E. onukii.
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