Complex assembly, crystallization and preliminary X-ray crystallographic analysis of duck MHC class I complexed with a TUMV viral peptide
文献类型： 外文期刊
作者： Liu, Zixin 1 ; Xie, Xiaoli 1 ; Li, Zhuolin 1 ; Zhang, Lin 2 ; Zhang, Nianzhi 1 ;
作者机构： 1.China Agr Univ, Coll Vet Med, Dept Microbiol & Immunol, Beijing, Peoples R China
2.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Dis Control & Breeding, Jinan, Shandong, Peoples R China
关键词： Duck; MHC class I; Peptide; TMUV; Crystallization; Complex assembly
期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）
ISSN： 0034-5288
年卷期： 2020 年 132 卷
页码：
收录情况： SCI
摘要： The CTL immune response mediated by MHC I plays an important role in duck anti-TMUV infection. This study reports the expression, purification and crystallization of a complex of duck MHC class I molecules Anpl-UAA*SD, duck beta 2-microglobulin (Anpl-beta 2m) and the polypeptide LRKRQLTVL (LRK9) derived from Tembusu virus (TMUV) NS3. The crystal diffraction resolution is 1.50 angstrom and belongs to the P6(2) space group, and the unit cell parameters are a = 82.468, b = 82.468, c = 112.507. The Matthew's constant is calculated to be 2.32 angstrom(3) Da(-1), and an asymmetric unit contains a complex molecule with a solvent content of 47%. The research lays the foundation for the structure of immune molecules about duck anti-TMUV research.
- 相关文献
作者其他论文 更多>>
-
The impact of micropolymorphism in Anpl-UAA on structural stability and peptide presentation
作者：Tang, Ziche;Wang, Suqiu;Du, Liubao;Hu, Dongmei;Chen, Xiaoming;Zheng, Hanyin;Ding, Han;Chen, Shiwen;Zhang, Nianzhi;Zhang, Lin;Zhang, Lin
关键词：Micropolymorphism; Peptide presentation; RPLD-MS; Crystal structure
-
Plant iron status regulates ammonium-use efficiency through protein N-glycosylation
作者：Li, Guangjie;Zhang, Lin;Li, Guangjie;Wu, Jinlin;Wang, Zhaoyue;Wang, Meng;Shi, Weiming;Wu, Jinlin;Wang, Zhaoyue;Wang, Meng;Shi, Weiming;Kronzucker, Herbert J.;Shi, Weiming
关键词：
-
Important role of Bacillus subtilis as a probiotic and vaccine carrier in animal health maintenance
作者：Yuan, Chunmei;Ji, Xiang;Liu, Xinli;Zhu, Wenxing;Yuan, Chunmei;Ji, Xiang;Zhang, Yuyu;Ding, Luogang;Li, Jianda;Ren, Sufang;Liu, Fei;Chen, Zhi;Zhang, Lin;Yu, Jiang;Wu, Jiaqiang;Zhang, Yuyu;Yu, Jiang;Wu, Jiaqiang
关键词：Immunity; Intestinal flora balance; Probiotics; Spore surface display; Vaccine carriers
-
Evaluation of air pollution tolerance index of urban roadside young leaf and the correlation with its capturing capacity for water-insoluble fine particulate matters
作者：He, Chen;Zhang, Zhuofan;Wang, Qi;Zhang, Yupeng;Wei, Chenhui;Zhang, Lin;Zhang, Yilong;Yu, Hongqian;Li, Zhuo;Chang, Chunyan
关键词：Air pollution tolerance index (APTI); Roadside greenbelts; Relative water content; Ascorbic acid; Vehicular fine particles; Pollution tolerance
-
Dietary Resistant Starch Regulates Bile Acid Metabolism by Modulating the FXR/LRH-1 Signaling Pathway in Broilers
作者：Wang, Zhenxin;Zhan, Chunyan;Zhang, Yingying;Zhang, Lin;Xing, Tong;Zhao, Liang;Gao, Feng;Li, Jiaolong;Wang, Jianfei
关键词：resistant starch; bile acid metabolism; microorganism; FXR/LRH-1
-
Genomic prediction of yield performance among single-cross maize hybrids using a partial diallel cross design
作者：Luo, Ping;Wang, Houwen;Yang, Ruisi;Wang, Fei;Yong, Hongjun;Zhou, Zhiqiang;Li, Mingshun;Zhang, Degui;Han, Jienan;Chen, Yong;Zhang, Runze;Gao, Wenwei;Chen, Xiaoyu;Li, Wenjie;Hao, Zhuanfang;Fu, Junjie;Li, Xinhai;Luo, Ping;Zhang, Xuecai;Ni, Zhiyong;Zhang, Lin;Song, Wei;Wang, Liwei;Yang, Jie;Weng, Jianfeng;Meng, Zhaodong;Zhao, Meng
关键词：Maize; Genomic prediction; Prediction model; Genetic effects; Hybrid performance
-
Analysis of leaf forage value and screening of different populations of Pteroceltis tatarinowii, a rare and endemic species in China
作者：Gao, Yun;Cheng, Tian-Tian;Liu, Qing-Zhong;Qiao, Qian;Cheng, Tian-Tian;Zhang, Chun-Xiang;Yan, Yu;Zhang, Lin;Liu, Yan;Qiao, Qian
关键词：Pteroceltis tatarinowii; woody forage; crude protein (CP); forage value; population diversity
