文献类型： 外文期刊
作者： Liu, Gongyan 1 ; Bai, Liya 1 ; Li, Shu 2 ; Liu, Hongli 2 ; Zhu, Yanli 2 ; Sun, Haitao 1 ; Gao, Shuxia 1 ; Jiang, Wenxue 1 ;
作者机构： 1.Shandong Acad Agr Sci, Anim Husb & Vet Inst, Shandong Key Lab Anim Dis Control & Breeding, Jinan 251000, Peoples R China
2.Shandong Agr Univ, Coll Anim Sci & Technol, Shandong Prov Key Lab Anim Biotechnol & Dis Contr, Tai An 271018, Shandong, Peoples R China
关键词： Rex rabbit; Dermal papilla cell; Cell isolation; Growth characteristics
期刊名称：TISSUE & CELL （ 影响因子：2.466； 五年影响因子：2.235 ）
ISSN： 0040-8166
年卷期： 2020 年 65 卷
页码：
收录情况： SCI
摘要： Dermal papilla cells (DPCs) is the key dermal component of the hair follicle that directly regulates hair follicle development, growth and regeneration. Successfully isolated and cultured DPCs of Rex rabbit could provide a good model for the study of hair follicle development mechanism in vitro. Skin samples were collected from 30-day old Rex rabbits and separated by combination of Dispase II and Collagenase D, separation, culture, and purification of DPCs. The morphology of DPCs in vitro was observed and the growth curve was drawn, the number of DPCs presented progressive increase in a logarithmic model between the 4th day and the 7th day. The results of immune chemical and immune fluorescence shown that a smooth muscle actin (alpha-SMA) and versican were positive in cells. Growth character of the passages 3 (P3), P6, P9 and P12 DPCs were observed using MTT at 24 h, 48 h, 72 h, 96 h, 120 h and 144 h. The cell density of P12 was lower than P3 (P < 0.05); the flow cyto-metric analysis showed that DPCs at resting state/first gap (G0/G1) stage of P3 was higher than P12 (P < 0.05), and second gap/mitosis (G2/M) stage of P3 was lower than P12 (P < 0.05). However, the DPCs of P12 present triangular or short fusiform, retaining their unique aggregative growth characteristics. This results shown that the DPCs properties of P12 from Rex rabbits, still fit functional research in vitro. In conclusion, we successfully established the culturing condition of DPCs from Rex rabbits, and provide a material for studying the molecular mechanism of hair follicle development.
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