文献类型： 外文期刊

作者： Dai, Xiaoyan ¹ ; Wang, Yu ¹ ; Liu, Yan ¹ ; Wang, Ruijuan ¹ ; Su, Long ¹ ; Yin, Zhenjuan ⁵ ; Zhao, Shan ¹ ; Chen, Hao ¹ ; Zheng, Li ¹ ; Dong, Xiaolin ⁴ ; Zhai, Yifan ¹ ;

作者机构： 1.Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Nat Enemies Insects, Jinan 250100, Peoples R China

3.MARA CABI Joint Lab Biosafety Shandong Subctr, Jinan 250100, Peoples R China

4.Yangtze Univ, Coll Agr, Jingzhou 434023, Peoples R China

5.Guizhou Univ, Coll Agr, Guiyang 550025, Peoples R China

关键词： Aphidoletes aphidimyza; diapause induction; sensitive stage; transcriptome; carbohydrate metabolism

期刊名称：INSECTS （ 影响因子：3.0； 五年影响因子：3.1 ）

ISSN：

年卷期： 2024 年 15 卷 5 期

页码：

收录情况： SCI

摘要： Simple Summary: Aphidoletes aphidimyza (Rondani) is an important predatory insect, primarily preying on aphids. The effective control of diapause in A. aphidimyza is a crucial practical issue in the field of biological pest control and large-scale artificial insect breeding. Previous research has shown that, in the European strain of A. aphidimyza, diapause occurs during the larval stage in the cocoon, it has a day-long response, and it enters diapause when the day length decreases. In this study, we evaluated the effects of photoperiod and temperature on the incidence of diapause in A. aphidimyza. We found that the diapause occurs in the cocooning larvae stage, and the highest diapause rate was recorded at under 15 degrees C and 10L:14D photoperiod conditions. Transcriptome sequencing analysis showed that differentially expressed genes were mainly enriched in the glucose metabolism pathway. The aphidophagous gall midge, Aphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae), a dominant natural enemy of aphids, has been used as a biological control agent in many countries to control aphids in greenhouses. To identify key factors that induce diapause in A. aphidimyza, we evaluated the effects of photoperiod and temperature on the incidence of diapause in A. aphidimyza under laboratory conditions. The results showed that temperature and photoperiod had significant impacts on development and diapause in A. aphidimyza. Low temperatures and a short photoperiod inhibited development, while high temperatures and a long photoperiod promoted development. Temperatures above 20 degrees C and a photoperiod greater than 14 h prevented diapause in A. aphidimyza. However, the highest diapause rate was recorded at under 15 degrees C and 10L:14D photoperiod conditions. At 15 degrees C, the first to third larvae were sensitive to a short photoperiod at any stage, and a short photoperiod had a cumulative effect on diapause induction. The longer the larvae received short light exposure, the higher the diapause rate appeared to be. Transcriptome sequencing analysis at different stages of diapause showed that differentially expressed genes were mainly enriched in the glucose metabolism pathway. Physiological and biochemical analyses showed that diapausing A. aphidimyza reduced water content; accumulated glycogen, trehalose, sorbitol, and triglycerides; and gradually reduced trehalose and triglyceride contents in the body with the extension of diapause time. Glycogen may be used as a source of energy, but sorbitol is usually used as a cryoprotectant. This study provided results on aspects of diapause in A. aphidimyza, providing data and theoretical support for promoting its commercial breeding and in-depth research on the molecular mechanisms underlying diapause regulation.