Identification of Lipopeptide Iturin A Produced by Bacillus amyloliquefaciens NCPSJ7 and Its Antifungal Activities against Fusarium oxysporum f. sp. niveum
文献类型: 外文期刊
作者: Wang, Junhua 1 ; Qiu, Jiying 1 ; Yang, Xiaoyu 1 ; Yang, Jinyu 1 ; Zhao, Shuangzhi 1 ; Zhou, Qingxin 1 ; Chen, Leilei 1 ;
作者机构: 1.Shandong Acad Agr Sci, Inst Agrofood Sci & Technol, Minist Agr & Rural Affairs, Key Lab Novel Food Resources Proc,Key Lab Agropro, Jinan 250100, Peoples R China
关键词: Bacillus amyloliquefaciens; iturin A; ultrastructure; ergosterol; fungicidal activities; Fusarium oxysporum f. sp.; niveum
期刊名称:FOODS ( 影响因子:5.561; 五年影响因子:5.94 )
ISSN:
年卷期: 2022 年 11 卷 19 期
页码:
收录情况: SCI
摘要: Bacillus amyloliquefaciens NCPSJ7 showed potential fungicidal activities for the effective control of fungal infection. From the PCR test, the key genes (srfAA, sfp, fenD, bmyB, ituD, and ituC) were detected in B. amyloliquefaciens NCPSJ7. These genes were closely related to the lipopeptides (LPs) synthesis. Next, three LPs families were identified with liquid chromatography-mass spectrometry (LC/MS), including iturin A, fengycin A, and surfactin. After purification with C18, the main active antifungal compound was proven to be C14-iturin A by ESI-HRMS, which has significant activities against fungi. These results proved that C14-iturin A played an important role in inhibiting the growth of fungi for B. amyloliquefaciens NCPSJ7. Furthermore, the isolated LP could inhibit mycelial growth and conidia germination at 30 mu g/mL. SEM allowed us to observe that mycelial morphology and conidia germination were also affected. The mycelial ultrastructure TEM observations showed that the external electron-dense outer layer cell wall, which mainly consisted of glycoproteins, was affected. Furthermore, swollen mitochondria, enriched glycogen, and increased vacuoles were also found. LP also affected the intact wall and membranes, leading to their increased permeability, which was proved by propidium iodide (PI) staining and conductivity measurements. Meanwhile, the ergosterol, which has an affinity for iturin A, also increased. These results indicated that LP caused fungal dysfunction and membrane permeability increase, leading to fungal inhibition. Identifying and studying LPs is important in exploring the fungicidal activities of B. amyloliquefaciens, which promotes the use of B. amyloliquefaciens NCPSJ7 as a potential candidate for biocontrol.
- 相关文献
作者其他论文 更多>>
-
Quality maintenance mechanism of oxalic acid treatment in fresh-cut apple fruit during storage based on nontarget metabolomics analysis
作者:Wang, Wenliang;Cao, Ziming;Hou, Furong;Shi, Junyan;Chen, Leilei;Gong, Zhiqing;Wang, Yansheng;Cao, Ziming;Jiao, Jian;Wang, Yansheng
关键词:Oxalic acid; Fresh-cut apples; Browning; Metabolomics; Storage quality
-
The extraction of effective components and an antioxidant activity study of Tulipa edulis
作者:Zhang, Doudou;Zhao, Shuangzhi;Chen, Leilei;Zhang, Doudou;Yin, Tingting;Zhur, Olena;Xiao, Xun;He, Hailun;Xiao, Dong;Zhao, Shuangzhi;Chen, Leilei
关键词:Tulipa edulis; Oxidative stress; Purification; Antioxidant
-
Transcriptome and metabolome integrated analysis revealed the effects and potential mechanism of hydrogen peroxide on antioxidant system in postharvest broccoli
作者:Zhang, Yuxiao;Chen, Ying;Guo, Yanyin;Sun, Yupeng;Wang, Zhengli;Wang, Yunqiao;Guan, Lingxing;Wang, Liang;Zhou, Qingxin
关键词:Ascorbic acid; Broccoli; Glucosinolate; Glutathione; Multi-omics; Reactive oxygen species
-
Light calcium carbonate improves pullulan biosynthesis by Aureobasidium pullulans under high concentration of sugar
作者:Yang, Jinyu;Li, Xiwen;Zhao, Shuangzhi;Yuan, Wei;Zhou, Qingxin;Zhang, Yanhao;Qiu, Jiying;Wang, Junhua;Zhu, Qingjun;Yang, Xiaoyu;Jiang, Xiaoxiao;Chen, Leilei;Yuan, Wei;Zhou, Qingxin;Tian, Chengsen;Li, Xiwen;Tian, Chengsen;Chen, Leilei
关键词:Aureobasidium pullulans; Pullulan biosynthesis; Light CaCO 3; Osmophiles; Key biosynthetic enzymes
-
Biofilm-forming ability of Salmonella enterica strains of different serotypes isolated from multiple sources in China
作者:Liu, Xu;Jiang, Zhaoxu;Liu, Zijun;Li, Donghui;Liu, Zhenhai;Dong, Xiaorui;Yan, Shigan;Zhu, Liping;Cui, Daoshi;Chen, Leilei;Wang, Junwei
关键词:Salmonella enterica; Biofilm-forming ability; Serotype
-
High-level production of pullulan from high concentration of glucose by mutagenesis and adaptive laboratory evolution of Aureobasidium pullulans
作者:Li, Xiwen;Yu, Xiaobin;Li, Xiwen;Zhao, Shuangzhi;Chen, Leilei;Zhou, Qingxin;Qiu, Jiying;Xin, Xue;Zhang, Yanhao;Yuan, Wei;Yang, Jinyu;Chen, Leilei;Zhou, Qingxin;Yuan, Wei;Tian, Chengsen;Chen, Leilei;Zhou, Qingxin;Yuan, Wei;Tian, Chengsen
关键词:Pullulan; Aureobasidium pullulans; Adaptive laboratory evolution; High glucose concentration
-
Catalase-peroxidase StKatG is a bacterial manganese oxidase from endophytic Salinicola tamaricis
作者:Zhao, Guoyan;Wang, Wenjing;Duan, Guowen;Chang, Runlei;Chen, Zhuo;Zhang, Susu;Dai, Meixue;Yang, Guiwen;Zheng, Linlin;Chen, Leilei
关键词:Biogenic manganese oxides; Mn(II) oxidation; Manganese -oxidizing bacteria; Haem peroxidases; Endophytic bacteria