您好，欢迎访问山东省农业科学院文献资源数据库平台

Temporal Dynamics of Antibiotic Resistance Genes in Vegetable Greenhouse Soils Following Different Manure Applications

文献类型： 外文期刊

作者： Yuan, Xuexia 1 ; Zhang, Yong 3 ; Fan, Lixia 1 ; Wang, Wenbo 1 ; Wu, Yuanjuan 1 ;

作者机构： 1.Shandong Acad Agr Sci, Inst Agr Stand & Testing Technol Agriprod, Jinan, Peoples R China

2.Shandong Prov Key Lab Test Technol Food Qual & Sa, Jinan, Peoples R China

3.Shandong Inst Terr & Spatial Planning, Jinan, Peoples R China

关键词： Antibiotic resistance genes; Integron-integrase genes; Antibiotics; Soil bacterial community; Manure; Vegetable greenhouse

期刊名称：JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION （ 影响因子：3.61； 五年影响因子：3.581 ）

ISSN： 0718-9508

年卷期：

页码：

收录情况： SCI

摘要： The profiles of antibiotic resistance genes (ARGs) following manure application in vegetable greenhouse should be paid more attention due to its particular environment inside. Temporal dynamics of ARGs were investigated in two vegetable greenhouse soil after cattle manure (CM), swine manure (SM), and poultry manure (PM) application. Hopefully, the results could provide useful reference for safer manure application and respective policy formulation. Two plastic vegetable greenhouses with different cultivation histories located in Shandong province, where accounts for 50% of greenhouse cultivation in China, were selected to be applied with 3 types of manure, namely CM, SM, and PM. The temporal changes in abundance of ARGs, 2 integron-integrase (intI) genes, bacterial community composition, and concentration of antibiotics were analyzed. intI 2 was introduced into soil from all types of animal manure, and bla(TEM) was only from CM. Three manure applications all enriched the total abundances of 12 ARGs in soil throughout 28 days with SM being most significant. The changing patterns of individual ARGs after manure application were different, and mostly dependent on the manure types. The abundance of bacteria (Firmicutes, Actinobacteria, Synergistetes, and Fibrobacteres) was positively correlated with most of ARGs, and bacteria (Proteobacteria, Bacteroidetes, Deinococcus-Thermus, BRC1, and Epsilonbacteraeota) was positively correlated with specific ARGs, indicating these bacteria were potential hosts of specific ARGs. Contribution of intI genes (14.63%), and its interaction with bacterial community (22.16%) and antibiotics (14.07%) to ARGs variation highlighted the importance of intI genes in the spread and transfer of ARGs from manure to agricultural system. This study provided a direct evidence of ARGs introduction and enrichment in greenhouse soils by manure application. Proper measures should be taken to reduce the levels of antibiotic resistant bacteria, ARGs, intI genes, and associated risk before manure application into soil.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：山东省农业科学院文献资源数据库平台

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台