A highly sensitive aptasensor based on 3D-rGO/AuNPs for Hg2+ determination using HCR amplification strategy triggered by T-Hg2+-T
文献类型： 外文期刊
作者： Shao, Yangyang 1 ; Dong, Yanjie 1 ; Fan, Lixia 1 ; Xu, Weidong 4 ; Bin, Liu 1 ; Wang, Lei 1 ; Li, Dapeng 3 ; Zhao, Shancang 1 ;
作者机构： 1.Shandong Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Jinan, Shandong, Peoples R China
2.Shandong Prov Key Lab Test Technol Food Qual & Sa, Jinan, Shandong, Peoples R China
3.Shandong Agr Univ, Tai An, Shandong, Peoples R China
4.Yuncheng Inspect & Testing Ctr, Yuncheng, Shandong, Peoples R China
关键词： Aptasensor; Three-dimensional reduced graphene oxide; gold nanoparticles; Hybridization chain reaction; T-Hg2+-T; Toluidine blue
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.304； 五年影响因子：4.723 ）
ISSN： 0026-265X
年卷期： 2022 年 177 卷
页码：
收录情况： SCI
摘要： In this paper, we developed an electrochemical aptasensor with high sensitivity based on the stable T-Hg2+-T structure for Hg2+ determination. The three-dimensional reduced graphene oxide-modified gold nanoparticles (3D-rGO/AuNPs) was served as support material, which offered more immobilization sites for thiolated probe (P1). In the presence of Hg2+, P1 could interact with another probe (P2) by forming T-Hg2+-T structure. Further, the partial sequence of P2 that did not interact with P1 was employed as an initiator sequence, which resulted in the formation of the extended double-stranded DNA through the hybridization chain reaction (HCR) between two harpin DNA strands (HP1 and HP2). Toluidine blue (TB) was used to interact with the double-stranded DNA and bring in effective electrochemical responses. Under the optimal experimental conditions, the aptasensor exhibited pronounced electrochemical signals towards Hg2+ ranging from 10 fM to 50 nM, with a low detection limit of 3.3 fM. Besides, the aptasensor showed excellent performance in water samples for Hg2+ determination.
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