3-Monochloropropane-1,2-diol causes spermatogenesis failure in male rats via Sertoli cell dysfunction but not testosterone reduction
文献类型： 外文期刊
作者： Xing, Hanzhu 1 ; Chen, Shanbin 3 ; Wang, Xiaoli 4 ; Li, Jinwang 1 ; Ren, Fazheng 1 ;
作者机构： 1.China Agr Univ, Dept Nutr & Hlth, Key Lab Precis Nutr & Food Qual, Beijing 100083, Peoples R China
2.Qilu Univ Technol, Sch Food Sci & Engn, Shandong Acad Sci, Jinan 250353, Peoples R China
3.Shandong Acad Agr Sci, Inst Agr Prod Proc & Nutr Res, Jinan 250100, Peoples R China
4.Qilu Univ Technol, Shandong Anal & Test Ctr, Key Lab Appl Technol Sophisticated Analyt Instrum, Shandong Acad Sci, Jinan 250014, Peoples R China
关键词： 3-Monochloropropane-1,2-diol; Spermatogenesis; Sertoli cell; Androgen receptor; Paracrine factors
期刊名称：TOXICOLOGY LETTERS （ 影响因子：4.271； 五年影响因子：4.293 ）
ISSN： 0378-4274
年卷期： 2022 年 360 卷
页码：
收录情况： SCI
摘要： 3-Monochloropane-1,2-diol (3-MCPD), a common food contaminant, has been confirmed to impair male fertility, but the mechanism has not been fully clarified. This study systematically explored the spermatogenesis impairment induced by 3-MCPD in vivo and in vitro with a focus on Sertoli cells (SCs) and spermatogonial stem cells (SSCs). After adult male Sprague-Dawley rats were administered 36 and 72 mg/kg b.w./day 3-MCPD daily for 4 weeks, the total sperm concentration dramatically decreased by 28.9 % and 57.7 %, respectively, and obvious testicular seminiferous tubule atrophy was observed. 3-MPCD exposure decreased serum testosterone levels but not intratesticular testosterone levels and upregulated the expression of steroidogenesis enzymes in both rat testes and primary Leydig cells. 3-MCPD did not reduce the number and self-renewal marker PLZF+ of SSCs; however, it downregulated the key meiotic genes Stra8 and Rec8 in the rat testis but not in primary germ cells. Although SC counts were not affected, 3-MCPD downregulated androgen receptor (AR) in rat testes and primary SCs. In addition, 3-MCPD downregulated p-CREB (transcription factor of AR), paracrine meiosis regulators Nrg1 and Nrg3 and retinoic acid synthetase Aldh1a1 in primary SCs. In summary, 3-MCPD caused impairment of spermatogenesis by inhibiting secretion of meiosis regulators and disturbing testosterone signalling in SCs.
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