文献类型： 外文期刊

作者： Yu, Shasha ¹ ; Dong, Xiaodan ² ; Ma, Rongjie ¹ ; Ji, Haiyu ¹ ; Yu, Juan ¹ ; Liu, Anjun ¹ ;

作者机构： 1.Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Key Lab Food Nutr & Safety, Minist Educ, Tianjin 300457, Peoples R China

2.Shandong Acad Agr Sci, Jinan 250100, Peoples R China

3.Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Key Lab Food Nutr & Safety, Minist Educ, 29, 13th St, TEDA, Tianjin 300457, Peoples R China

关键词： Polygala tenuifolia Willd; Polysaccharide; Structural characteristic; Immunomodulatory activity

期刊名称：BIOORGANIC CHEMISTRY （ 影响因子：5.307； 五年影响因子：5.321 ）

ISSN： 0045-2068

年卷期： 2023 年 130 卷

页码：

收录情况： SCI

摘要： Polysaccharides from the Polygala tenuifolia Willd. have been shown multiple biological activities, however the structural feature and immunomodulatory activity are still rarely reported. In this study, a polysaccharide was obtained by purification, and its structural characteristics and immune activity were analyzed. The poly-saccharide was a homogeneous macromolecular polysaccharide with smooth flat flakes surface structure and molecular weight of 2.34 x 105 Da, and composed of Rha, Ara, Xyl, Man, Glc, Gal. Methylation and NMR an-alyses confirmed that the repeating unit of polysaccharide was [-> 3)-alpha-Araf-(1 -> 3)-alpha-Araf-(1 -> 5)-alpha-Araf-(1 -> 5)-alpha-Araf-(1 -> 3)-alpha-Araf-(1 -> ]n, and the side chain was alpha-Araf-(1 -> 6)-beta-Galp-(1 -> 6)-beta-Glcp-(1 -> 6)-alpha-Manp- (1 ->, which was attached to the C3 of -> 3,5)-alpha-Araf-(1 ->. In vitro, the RAW 264.7 cells were co-cultivated with LPS and polysaccharide, and the results revealed that the polysaccharide can promote cell proliferation, activate effectors to release cytokines (TNF-alpha, IL-6, IL-1 beta), and then activate macrophages for immune activity. Therefore, we can infer that the polysaccharide might regard as a potential immunomodulator.